Anti-SNX27 antibody [1C6] (ab77799)
![Anti-SNX27 antibody [1C6] (ab77799)](https://www.abcam.com/ps/products/77/ab77799/Images/SNX27-Primary-antibodies-ab77799-3.jpg "Anti-SNX27 antibody [1C6] (ab77799)")
Key features and details
- Mouse monoclonal [1C6] to SNX27
- Suitable for: Flow Cyt, WB, IP
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-SNX27 antibody [1C6]
See all SNX27 primary antibodies -
Description
Mouse monoclonal [1C6] to SNX27 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIP HumanWB Human
-
Immunogen
Fusion protein corresponding to Human SNX27 aa 1-300 (N terminal).
-
Epitope
N-terminal amino acids 1-267. -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: HeLa; A431; HEK293; A549; MDA-MB-231.
-
General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
1C6 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Research areas
Images
-
Western blot - Anti-SNX27 antibody [1C6] (ab77799)All lanes : Anti-SNX27 antibody [1C6] (ab77799) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lane 5 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 15 minutesBlocking: 5% Milk in TBS-T.
Two bands are observed very close together, at around 60-kDa. There are four reported isoforms of the SNX27 protein (Swissprot). We hypothesise that this antibody detects both isoform 1 and isoform 2, which are 61-kDa and 60-kDa respectively. As the immunogen is derived from the N-terminal region of SNX27, the antibody is not expected to react with isoforms 3 or 4.
-
![Flow Cytometry - Anti-SNX27 antibody [1C6] (ab77799)](https://www.abcam.com/ps/products/77/ab77799/Images/SNX27-Primary-antibodies-ab77799-7.jpg)
Flow Cytometry - Anti-SNX27 antibody [1C6] (ab77799)Overlay histogram showing Jurkat cells stained with ab77799 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77799, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
-
![Immunoprecipitation - Anti-SNX27 antibody [1C6] (ab77799)](https://www.abcam.com/ps/products/77/ab77799/Images/SNX27-Primary-antibodies-ab77799-8.jpg)
Immunoprecipitation - Anti-SNX27 antibody [1C6] (ab77799)SNX27 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 5µg of Mouse monoclonal to SNX27 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab77799.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
Band: 61kDa: SNX27.
