Anti-Caveolin-3 antibody (ab87770)
Key features and details
- Goat polyclonal to Caveolin-3
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG
Overview
-
Product name
Anti-Caveolin-3 antibody
See all Caveolin-3 primary antibodies -
Description
Goat polyclonal to Caveolin-3 -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Pig
Predicted to work with: Cow, Dog
-
Immunogen
Synthetic peptide corresponding to Human Caveolin-3 aa 5-18 (N terminal) (Cysteine residue).
Sequence:EHTDLEAQIVKDIH
Database link: P56539 -
Positive control
- Recombinant Human Caveolin-3 protein (ab114264) can be used as a positive control in WB. Human, mouse and rat heart lysates.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Caveolin-3 antibody (ab87770)All lanes : Anti-Caveolin-3 antibody (ab87770) at 0.1 µg/ml
Lane 1 : pig skeletal muscle tissue lysate
Lane 2 : pig heart tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 17 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?Detected by chemiluminescence.
-
Western blot - Anti-Caveolin-3 antibody (ab87770)All lanes : Anti-Caveolin-3 antibody (ab87770) at 0.3 µg/ml
Lane 1 : mouse heart tissue lysate
Lane 2 : rat heart tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 17 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?Detected by chemiluminescence.
-
Western blot - Anti-Caveolin-3 antibody (ab87770)Anti-Caveolin-3 antibody (ab87770) at 0.3 µg/ml + human heart lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 17 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
Exposure time: 1 hourDetected by chemiluminescence.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody (ab87770)ab87770 (3.8µg/ml) staining of paraffin embedded Human Skeletal Muscle shows peripheral staining of fibres. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
