All lanes : Anti-Caveolin-3 antibody [EPR11083] (ab171752) at 1/1000 dilution

Lane 1 : Human skeletal muscle lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Mouse muscle lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 17 kDa

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Caveolin-3 with ab171752 at 1/4000 dilution (0.131 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on human skeletal muscle (PMID: 11431690). The section was incubated with ab171752 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling Caveolin-3 with ab171752 at 1/4000 dilution (0.131 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous and weak cytoplasmic staining on human cardiac muscle (PMID: 28356340). The section was incubated with ab171752 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Western blot analysis on immunoprecipitation pellet from Human fetal heart lysate labeling Caveolin 3 using ab171752 at 1/10 dilution.

Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling Caveolin-3 with ab171752 at 1/4000 dilution (0.131 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous and weak cytoplasmic staining on rat cardiac muscle (PMID: 28356340). The section was incubated with ab171752 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling Caveolin-3 with ab171752 at 1/4000 dilution (0.131 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous and weak cytoplasmic staining on mouse skeletal muscle (PMID: 11431690). The section was incubated with ab171752 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling Caveolin-3 with ab171752 at 1/4000 dilution (0.131 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous and cytoplasmic staining on mouse cardiac muscle (PMID: 28356340). The section was incubated with ab171752 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).