Anti-CD147 antibody [OTI9B10] (ab119020)
![Anti-CD147 antibody [OTI9B10] (ab119020)](https://www.abcam.com/ps/products/119/ab119020/Images/ab119020-446554-anti-cd147-antibody-oti9b10-western-blot-mps-a549-wt-mps-a549-cd147bsg-knockout-raji-jurkat.jpg "Anti-CD147 antibody [OTI9B10] (ab119020)")
Key features and details
- Mouse monoclonal [OTI9B10] to CD147
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-CD147 antibody [OTI9B10]
See all CD147 primary antibodies -
Description
Mouse monoclonal [OTI9B10] to CD147 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF African green monkeyWB Human
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Immunogen
Recombinant full length protein corresponding to Human CD147.
Database link: NP_001719 -
Positive control
- WB: A549, Raji and Jurkat cell lysates; HEK293T cell lysate transfected with pCMV6-ENTRY CD147 cDNA ICC/IF: COS7 cells transiently transfected with pCMV6-ENTRY CD147
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General notes
The clone number has been updated from 9B10 to OTI9B10, both clone numbers name the same clone.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 50% Glycerol, PBS -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant by affinity chromatography -
Clonality
Monoclonal -
Clone number
OTI9B10 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-CD147 antibody [OTI9B10] (ab119020)All lanes : Anti-CD147 antibody [OTI9B10] (ab119020) at 1/2000 dilution
Lane 1 : Wild-type A549 cell lysate
Lane 2 : BSG knockout A549 cell lysate
Lane 3 : Raji cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 30 µg per lane.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 55-70 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab119020 observed at 55-70 kDa. Red - loading control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55kDa.
ab119020 was shown to react with CD147 in wild-type A549 cells in western blot with loss of signal observed in BSG knockout cell line ab273748 (knockout cell lysate ab275500). Wild-type and BSG knockout A549 cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab119020 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at a 1 in 2000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-CD147 antibody [OTI9B10] (ab119020)](https://www.abcam.com/ps/products/119/ab119020/Images/CD147-Primary-antibodies-ab119020-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD147 antibody [OTI9B10] (ab119020)ab119020, at a 1/100 dilution, staining CD147 in COS7 cells transiently transfected with pCMV6-ENTRY CD147 by Immunofluorescence. -
![Western blot - Anti-CD147 antibody [OTI9B10] (ab119020)](https://www.abcam.com/ps/products/119/ab119020/Images/CD147-Primary-antibodies-ab119020-1.jpg)
Western blot - Anti-CD147 antibody [OTI9B10] (ab119020)All lanes : Anti-CD147 antibody [OTI9B10] (ab119020) at 1/2000 dilution
Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY CD147 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 42 kDa
