Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

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Anti-CD147 antibody [EPR18008-8] (ab188190)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18008-8] to CD147
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP, Sandwich ELISA
Reacts with: Mouse, Recombinant fragment

Product name

Anti-CD147 antibody [EPR18008-8]
See all CD147 primary antibodies
Description

Rabbit monoclonal [EPR18008-8] to CD147
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Mouse
ICC/IF	Mouse
IHC-P	Mouse
IP	Mouse
sELISA	Recombinant fragment
WB	Mouse Recombinant fragment

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse CD147 recombinant protein fragment; RAW 264.7, WEHI-3 and bEND.3 whole cell lysates; mouse liver lysate. IHC-P: Mouse intestine tissue. ICC/IF: WEHI-231 and bEND.3 cells. Flow Cyt: Mouse thymocytes. IP: RAW 264.7 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18008-8
Isotype

IgG
Research areas

Western blot - Anti-CD147 antibody [EPR18008-8] (ab188190)

All lanes : Anti-CD147 antibody [EPR18008-8] (ab188190) at 1/5000 dilution

Lane 1 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 2 : Mouse liver lysate
Lane 3 : WEHI-3 (Mouse leukemia cell line) whole cell lysate
Lane 4 : bEnd.3 (Mouse brain endothelioma cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 42 kDa
Observed band size: 45-55 kDa
why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times: Lane 1/2: 3 minutes; Lane 3: 10 seconds; Lane 4: 1 second.

The expression profile observed is consistent with what has been described in the literature (PMID: 16721788; 23966157).
Immunocytochemistry/ Immunofluorescence - Anti-CD147 antibody [EPR18008-8] (ab188190)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized bEND.3 (Mouse brain capillary endothelial cell line) cells labeling CD147 with ab188190 at 1/250 dilution, followed by by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cell membrane staining on bEND.3 cell line. The nuclear counter stain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

The negative controls are as follows:
-ve control 1: ab188190 at 1/250 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD147 antibody [EPR18008-8] (ab188190)

Immunohistochemical analysis of paraffin-embedded mouse intestine tissue labeling CD147 with ab188190 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining on mouse intestine is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-CD147 antibody [EPR18008-8] (ab188190)

CD147 was immunoprecipitated from 1 mg of RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate with ab188190 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab188190 at 1/5000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: RAW 264.7 whole cell lysate 10µg (Input).

Lane 2: ab188190 IP in RAW 264.7 whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab188190 in RAW 264.7 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 1 second.
Flow Cytometry - Anti-CD147 antibody [EPR18008-8] (ab188190)

Flow cytometric analysis of fresh mouse thymocytes labeling CD147 with ab188190 at 1/200 dilution (red) compared with Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor^® 488) at 1/500 dilution was used as the secondary antibody.
Sandwich ELISA - Anti-CD147 antibody [EPR18008-8] (ab188190)

Standard Curve for CD147 (Analyte: Recombinant mouse CD147 protein) dilution range 0-500 pg/mL using Capture antibody at 0.2 ug/mL and Detector Antibody at 0.5 ug/mL. Secondary antibody: Peroxidase Streptavidin SA-HRP at 1/20000 dilution. Concentration of ab188190 may vary from lot to lot; please use this curve as guideline.

Washing buffer: 1X PBST
Blocking/Diluting buffer and concentration: 1% BSA/PBS
Immunocytochemistry/ Immunofluorescence - Anti-CD147 antibody [EPR18008-8] (ab188190)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized WEHI-231 (Mouse B Cell Lymphoma cell line) cells labeling CD147 with ab188190 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cell membrane staining on WEHI-231 cell line. The nuclear counter stain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

The negative controls are as follows:
-ve control 1: ab188190 at 1/250 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Western blot - Anti-CD147 antibody [EPR18008-8] (ab188190)

Anti-CD147 antibody [EPR18008-8] (ab188190) at 1/5000 dilution + Mouse CD147 recombinant protein fragment at 0.002 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 42 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

Recombinant protein fragment Mouse CD147 contains aa140-325 with a His-Tag®. It was made in house
Anti-CD147 antibody [EPR18008-8] (ab188190)

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