Human CD105 ELISA Kit (ab217773)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 11.1 pg/ml
- Range: 78.125 pg/ml - 5000 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CD105 ELISA Kit
See all CD105 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Human serum 3 1.8% Inter-assay Sample n Mean SD CV% Human serum 5 3% -
Sample type
Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
11.1 pg/ml -
Range
78.125 pg/ml - 5000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 107 84% - 120% Cell culture extracts 103 99% - 110% Tissue Extracts 106 96% - 119% Cell culture media 107 95% - 116% Hep Plasma 88 71% - 113% EDTA Plasma 100 84% - 119% Cit plasma 80 74% - 84% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human CD105 ELISA Kit (ab217773) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD105 protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD105 with 11.1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
CD105 is a major glycoprotein of vascular endothelium. CD105 is involved in the regulation of angiogenesis. It may play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. CD105 acts as TGF-beta co-receptor and is involved in the TGF-beta/BMP signaling cascade. It is required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and it modulates TGF-beta1 signaling through SMAD3. CD105 is a homodimer that forms a heteromeric complex with the signaling receptors for transforming growth factor-beta: TGFBR1 and/or TGFBR2. It is able to bind TGF-beta 1, and 3 efficiently and TGF-beta 2 less efficiently. Circulating soluble form of CD105 is elevated in patients with preeclampsia, atherosclerosis and certain cancers including breast, colorectal, and myeloid malignancies.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human CD105 Capture Antibody 1 x 600µl 10X Human CD105 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Denaturant 1 x 500µl Human CD105 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. -
Tissue specificity
Endoglin is restricted to endothelial cells in all tissues except bone marrow. -
Involvement in disease
Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- AI528660
- AI662476
- CD 105
see all -
Database links
- Entrez Gene: 2022 Human
- Omim: 131195 Human
- SwissProt: P17813 Human
- Unigene: 76753 Human
Images
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Other - Human CD105 ELISA Kit (ab217773)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human CD105 standard curve comparison dataStandard curve comparison between human CD105 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.
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Example of human CD105 standard curve in Sample Diluent NS + Enhancer.Background-subtracted data values (mean +/- SD) are graphed.
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Example of human CD105 standard curve in 1X Cell Extraction Buffer PTR + Enhancer.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native CD105 in human serum and plasma samples.The concentrations of CD105 were measured in duplicates, interpolated from the CD105 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 2.225%, plasma (citrate) 2.225%, plasma (heparin) 2.225% and plasma (EDTA) 2.225%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD105 concentration was determined to be 94,646 pg/mL in neat serum, 71,971 pg/mL in neat plasma (citrate), 75,408 pg/mL in neat plasma (heparin), and 76,842 pg/mL in neat plasma (EDTA).
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Interpolated concentrations of native CD105 in HUVEC cell extract, human placenta tissue extract and human heart tissue extract.Interpolated concentrations of native CD105 in HUVEC cell extract based on a 40 µg/mL extract load, human placenta tissue extract based on a 90 µg/mL extract load, and human heart tissue extract based on a 60 µg/mL extract load. The concentrations of CD105 were measured in duplicate and interpolated from the CD105 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD105 concentration was determined to be 2548 pg/mL in HUVEC cell extract, 542.9 pg/mL in placenta tissue extract, and 528.1 pg/mL in heart tissue extract.
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Serum from ten individual healthy female human donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD105 concentration was determined to be 61,333 pg/mL with a range of 30,103 – 91,256 pg/mL.
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Serum levels of CD105 are increased during the third trimester of pregnancy, especially in women with pre-eclampsia.Serum from 10 healthy (not pregnant) female individuals, 5 healthy females in the third trimester of pregnancy (pooled), and 1 individual female in the third trimester showing signs of mild pre-eclampsia were tested for levels of CD105. The concentrations of CD105 were measured from serum in samples in 3 different dilutions in duplicates and interpolated from the CD105 standard curve. The interpolated values are plotted (mean +/- SD, n=3). The mean CD105 concentration was determined to be 61,333 pg/mL in healthy female donor serum, 30,8945 pg/mL in healthy pregnant donor serum, and 703,119 pg/mL in pre-eclampsia patient serum.
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Sandwich ELISA - Human CD105 ELISA Kit (ab217773)To learn more about the advantages of recombinant antibodies see here.
