Anti-CD147 antibody [VJ1/9] (ab91147)
![Anti-CD147 antibody [VJ1/9] (ab91147)](https://www.abcam.com/ps/products/91/ab91147/Images/ab91147-3-Anti-CD147-antibody-VJ19-Human-A549-cells-flow-cytometry.jpg "Anti-CD147 antibody [VJ1/9] (ab91147)")
Key features and details
- Mouse monoclonal [VJ1/9] to CD147
- Suitable for: Flow Cyt
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD147 antibody [VJ1/9]
See all CD147 primary antibodies -
Description
Mouse monoclonal [VJ1/9] to CD147 -
Host species
Mouse -
Specificity
This antibody recognizes 50-60 kDa cell surface protein which is the Human homologue of Rat OX-47 antigen. The antibody recognizes the 118 kDa cell surface glycoprotein CD146, also known as MUC18, Mel-CAM and S-endo. CD146 is a member of the immunoglobulin superfamily, expressed by all endothelial cells and by melanoma cells. -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human
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Immunogen
Recombinant full length protein.
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Positive control
- Flow Cyt: A549 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified (pass through 0.22 µm filter) -
Clonality
Monoclonal -
Clone number
VJ1/9 -
Isotype
IgG1 -
Research areas
Images
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Flow Cytometry - Anti-CD147 antibody [VJ1/9] (ab91147)
Flow cytometry overlay histogram showing wild-type A549 (green line) and BSG knockout A549 cells (ab273748) stained with ab91147 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab91147) (1x106 in 100μl at 10 μg/ml) for 30 min at 4°C.
The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150117) was used at 1/2000 for 30 min at 4°C.
Isotype control antibody was mouse IgG1κ (ab170190) used at the same concentration and conditions as the primary antibody (wild-type A549 - black line; BSG knockout A549 - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).
Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.
