Anti-STIP1/STI1 antibody (ab228711)
Key features and details
- Rabbit polyclonal to STIP1/STI1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-STIP1/STI1 antibody
See all STIP1/STI1 primary antibodies -
Description
Rabbit polyclonal to STIP1/STI1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human STIP1/STI1 (internal sequence). The exact sequence is proprietary.
Database link: STIP1 -
Positive control
- WB: Jurkat, Raji, K562 and THP-1 whole cell lysates. IHC-P: BT43 xenograft tissue. ICC/IF: HeLa cells.
-
General notes
This product was previously labelled as STIP1
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-STIP1/STI1 antibody (ab228711)All lanes : Anti-STIP1/STI1 antibody (ab228711) at 1/10000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 4 : THP-1 (human monocytic leukemia cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 63 kDa7.5% SDS-PAGE gel.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIP1/STI1 antibody (ab228711)Paraffin-embedded BT483 xenograft tissue stained for STIP1/STI1 using ab228711 at 1/500 dilution in immunohistochemical analysis.
-
Immunocytochemistry/ Immunofluorescence - Anti-STIP1/STI1 antibody (ab228711)Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for STIP1/STI1 (green) using ab228711 at 1/500 dilution in ICC/IF.
Alpha-tubulin filaments were labeled with an anti-Alpha-tubulin antibody at 1/2000 dilution (red).
