Recombinant Human STIP1/STI1 protein (His tag) (ab256160)
Key features and details
- Expression system: Escherichia coli
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant Human STIP1/STI1 protein (His tag)
See all STIP1/STI1 proteins and peptides -
Biological activity
4uM ab256160, when added to 2uM Aha1-activated HSP90 (2uM; His-tagged HSP90 beta) in 33mM Hepes pH7.2, 30mM NaCl, 5mM MgCl2, 1mM DTT, 1.5mM ATP in a 100ul reaction at 37 degrees C, eliminated all Aha1-mediated ATPase stimulation as well as intrinsic HSP90 ATPase activity. (This is an enzyme-linked ATP regeneration assay tracking loss of NADH absorbance at 340nm).
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Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MGHHHHHHMEQVNELKEKGNKALSVGNIDDALQCYSEAIKLDPHNHVLYS NRSAAYAKKGDYQKAYEDGCKTVDLKPDWGKGYSRKAAALEFLNRFEEAK RTYEEGLKHEANNPQLKEGLQNMEARLAERKFMNPFNMPNLYQKLESDPR TRTLLSDPTYRELIEQLRNKPSDLGTKLQDPRIMTTLSVLLGVDLGSMDE EEEIATPPPPPPPKKETKPEPMEEDLPENKKQALKEKELGNDAYKKKDFD TALKHYDKAKELDPTNMTYITNQAAVYFEKGDYNKCRELCEKAIEVGREN REDYRQIAKAYARIGNSYFKEEKYKDAIHFYNKSLAEHRTPDVLKKCQQA EKILKEQERLAYINPDLALEEKNKGNECFQKGDYPQAMKHYTEAIKRNPK DAKLYSNRAACYTKLLEFQLALKDCEECIQLEPTFIKGYTRKAAALEAMK DYTKAMDVYQKALDLDSSCKEAADGYQRCMMAQYNRHDSPEDVKRRAMAD PEVQQIMSDPAMRLILEQMQKDPQALSEHLKNPVIAQKIQKLMDVGLIAI R -
Predicted molecular weight
64 kDa including tags -
Amino acids
1 to 543 -
Tags
His tag N-Terminus -
Additional sequence information
NP_006810.1
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C.
Constituents: 0.48% HEPES, 0.46% Sodium chloride, 10% Glycerol (glycerin, glycerine)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Recombinant Human STIP1/STI1 protein (His tag) (ab256160)
SDS-PAGE - Recombinant Human STIP1/STI1 protein (His tag) (ab256160)
