Anti-STIP1/STI1 antibody [EPR6606] - BSA and Azide free (ab248167)
![Anti-STIP1/STI1 antibody [EPR6606] - BSA and Azide free (ab248167)](https://www.abcam.com/ps/products/248/ab248167/Images/ab248167-414428-anti-stip1sti1-antibody-epr6606-bsa-and-azide-free-western-blot-hap1-wt-hap1-stip1-knockout-a431-hek293.jpg "Anti-STIP1/STI1 antibody [EPR6606] - BSA and Azide free (ab248167)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6606] to STIP1/STI1 - BSA and Azide free
- Suitable for: IP, IHC-P, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-STIP1/STI1 antibody [EPR6606] - BSA and Azide free
See all STIP1/STI1 primary antibodies -
Description
Rabbit monoclonal [EPR6606] to STIP1/STI1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC or ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HAP1, A431, HEK293, BxPC-3, Jurkat, HeLa, and HepG2 cell lysates. IHC-P: Human ovarian carcinoma tissue.
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General notes
ab248167 is the carrier-free version of ab126753 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab248167 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as STIP1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR6606 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-STIP1/STI1 antibody [EPR6606] - BSA and Azide free (ab248167)All lanes : Anti-STIP1/STI1 antibody [EPR6606] (ab126753) at 1/1000 dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : STIP1 knockout HAP1 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : HEK-293 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 63 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab126753).
Lanes 1 - 4: Merged signal (red and green). Green - ab126753 observed at 63 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab126753 was shown to react with STIP1/STI1 in wild-type HAP1 cells in western blot with loss of signal observed in STIP1 knockout sample. Wild-type and STIP1 knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab126753 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIP1/STI1 antibody [EPR6606] - BSA and Azide free (ab248167)](https://www.abcam.com/ps/products/248/ab248167/Images/ab248167-2-anti-stip1-sti1-antibody-epr6606-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIP1/STI1 antibody [EPR6606] - BSA and Azide free (ab248167)This data was developed using ab126753, the same antibody clone in a different buffer formulation. ab126753, at 1/50 dilution, staining STIP1/STI1 in paraffin-embedded Human ovarian carcinoma tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-STIP1/STI1 antibody [EPR6606] - BSA and Azide free (ab248167)](https://www.abcam.com/ps/products/248/ab248167/Images/ab248167-405424-anti-stip1-sti1-antibody-epr6606-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-STIP1/STI1 antibody [EPR6606] - BSA and Azide free (ab248167)All lanes : Anti-STIP1/STI1 antibody [EPR6606] (ab126753) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : BxPC-3 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution
Predicted band size: 63 kDaThis data was developed using ab126753, the same antibody clone in a different buffer formulation.
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![Immunoprecipitation - Anti-STIP1/STI1 antibody [EPR6606] - BSA and Azide free (ab248167)](https://www.abcam.com/ps/products/248/ab248167/Images/ab248167-399685-anti-stip1-sti1-antibody-epr6606-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-STIP1/STI1 antibody [EPR6606] - BSA and Azide free (ab248167)This data was developed using ab126753, the same antibody clone in a different buffer formulation.
ab126753 immunoprecipitating STIP1/STI1 in Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at a dilution of 1/30.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes : Anti-STIP1/STI1 antibody [EPR6606] (ab126753) at 1/1000 dilution
Lane 1 : IP using ab126753 in Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution
Observed band size: 63 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
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![Anti-STIP1/STI1 antibody [EPR6606] - BSA and Azide free (ab248167)](https://www.abcam.com/ps/products/248/ab248167/Images/ab248167-5-benefits-of-recombinant-antibodies.png)
Anti-STIP1/STI1 antibody [EPR6606] - BSA and Azide free (ab248167)
