Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-STIP1/STI1 antibody [EPR6606] - BSA and Azide free (ab248167)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6606] to STIP1/STI1 - BSA and Azide free
  • Suitable for: IP, IHC-P, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-STIP1/STI1 antibody [EPR6606] - BSA and Azide free
    See all STIP1/STI1 primary antibodies

  • Description

    Rabbit monoclonal [EPR6606] to STIP1/STI1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC or ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HAP1, A431, HEK293, BxPC-3, Jurkat, HeLa, and HepG2 cell lysates. IHC-P: Human ovarian carcinoma tissue.

  • General notes

    ab248167 is the carrier-free version of ab126753 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab248167 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as STIP1

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-STIP1/STI1 antibody [EPR6606] - BSA and Azide free (ab248167)
    Western blot - Anti-STIP1/STI1 antibody [EPR6606] - BSA and Azide free (ab248167)
    All lanes : Anti-STIP1/STI1 antibody [EPR6606] (ab126753) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 cell lysate
    Lane 2 : STIP1 knockout HAP1 cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : HEK-293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 63 kDa
    Observed band size: 63 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab126753).

    Lanes 1 - 4: Merged signal (red and green). Green - ab126753 observed at 63 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab126753 was shown to react with STIP1/STI1 in wild-type HAP1 cells in western blot with loss of signal observed in STIP1 knockout sample. Wild-type and STIP1 knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab126753 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIP1/STI1 antibody [EPR6606] - BSA and Azide free (ab248167)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIP1/STI1 antibody [EPR6606] - BSA and Azide free (ab248167)

    This data was developed using ab126753, the same antibody clone in a different buffer formulation. ab126753, at 1/50 dilution, staining STIP1/STI1 in paraffin-embedded Human ovarian carcinoma tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-STIP1/STI1 antibody [EPR6606] - BSA and Azide free (ab248167)
    Western blot - Anti-STIP1/STI1 antibody [EPR6606] - BSA and Azide free (ab248167)
    All lanes : Anti-STIP1/STI1 antibody [EPR6606] (ab126753) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : BxPC-3 cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution

    Predicted band size: 63 kDa



    This data was developed using ab126753, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-STIP1/STI1 antibody [EPR6606] - BSA and Azide free (ab248167)
    Immunoprecipitation - Anti-STIP1/STI1 antibody [EPR6606] - BSA and Azide free (ab248167)

    This data was developed using ab126753, the same antibody clone in a different buffer formulation.

    ab126753 immunoprecipitating STIP1/STI1 in Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at a dilution of 1/30.

    Blocking and dilution buffer: 5% NFDM/TBST.



    All lanes : Anti-STIP1/STI1 antibody [EPR6606] (ab126753) at 1/1000 dilution

    Lane 1 : IP using ab126753 in Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
    Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution

    Observed band size: 63 kDa
    why is the actual band size different from the predicted?


    Exposure time: 2 minutes
  • Anti-STIP1/STI1 antibody [EPR6606] - BSA and Azide free (ab248167)
    Anti-STIP1/STI1 antibody [EPR6606] - BSA and Azide free (ab248167)

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