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Astana Biomed Group, an authorized Abcam distributor in Central Asia

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Signal Transduction Cytoskeleton / ECM Cell Adhesion Cell Adhesion Molecules Vascular

Anti-VCAM1 antibody [EPR5038(2)] - BSA and Azide free (ab249790)

Anti-VCAM1 antibody [EPR5038(2)] - BSA and Azide free (ab249790)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5038(2)] to VCAM1 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-VCAM1 antibody [EPR5038(2)] - BSA and Azide free
    See all VCAM1 primary antibodies
  • Description

    Rabbit monoclonal [EPR5038(2)] to VCAM1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab249790 is the carrier-free version of ab174279. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249790 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5038(2)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cell Adhesion Molecules
    • Vascular
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cell Adhesion Molecules
    • Endothelial
    • Neuroscience
    • Neurology process
    • Neural Signal Transduction
    • Stem Cells
    • Mesenchymal Stem Cells
    • Surface Molecules
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Other
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Cell adhesion molecules
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Adhesion molecules ELISA kits
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Atherosclerotic proteins ELISA kits
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers

Images

  • Western blot - Anti-VCAM1 antibody [EPR5038(2)] - BSA and Azide free (ab249790)
    Western blot - Anti-VCAM1 antibody [EPR5038(2)] - BSA and Azide free (ab249790)
    All lanes : Anti-VCAM1 antibody [EPR5038(2)] (ab174279) at 1/1000 dilution

    Lane 1 : HUVEC (Human umbilical vein endothelial cell) whole cell lysate
    Lane 2 : HUVEC treated with 10 ng/ml TNF-a for 16 h, whole cell lysate
    Lane 3 : bEND.3 (Mouse brain endothelioma) whole cell lysate
    Lane 4 : bEND.3 treated with 10 µg/ml LPS for 24 h, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 81 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab174279, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-VCAM1 antibody [EPR5038(2)] - BSA and Azide free (ab249790)
    Western blot - Anti-VCAM1 antibody [EPR5038(2)] - BSA and Azide free (ab249790)
    All lanes : Anti-VCAM1 antibody [EPR5038(2)] (ab174279) at 1/1000 dilution

    Lane 1 : Hut-78 (Human Sezary syndrome cutaneous T lymphocyte) whole cell lysate
    Lane 2 : SK-OV-3 (Human ovarian cancer epithelial cell) whole cell lysate
    Lane 3 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 4 : J774A.1 (Mouse reticulum cell sarcoma monocyte macrophage) whole cell lysate
    Lane 5 : LADMAC (Mouse bone marrow monocyte macrophage) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 81 kDa
    Observed band size: 100 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    This data was developed using ab174279, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-VCAM1 antibody [EPR5038(2)] - BSA and Azide free (ab249790)
    Western blot - Anti-VCAM1 antibody [EPR5038(2)] - BSA and Azide free (ab249790)
    All lanes : Anti-VCAM1 antibody [EPR5038(2)] (ab174279) at 1/1000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : HuT-78 lysate
    Lane 3 : NIH3T3 lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 81 kDa



    This data was developed using ab174279, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-VCAM1 antibody [EPR5038(2)] - BSA and Azide free (ab249790)
    Immunoprecipitation - Anti-VCAM1 antibody [EPR5038(2)] - BSA and Azide free (ab249790)

    This data was developed using ab174279, the same antibody clone in a different buffer formulation.

    Immunoprecipitation. ab174279 at 1/1000 labeling VCAM1 immunoprecipitated from NIH3T3 cell lysate using ab174279 at 1/10.

  • Anti-VCAM1 antibody [EPR5038(2)] - BSA and Azide free (ab249790)
    Anti-VCAM1 antibody [EPR5038(2)] - BSA and Azide free (ab249790)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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