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Anti-VCAM1 antibody [EPR17010-83] - BSA and Azide free (ab232458)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17010-83] to VCAM1 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse

Overview

  • Product name

    Anti-VCAM1 antibody [EPR17010-83] - BSA and Azide free
    See all VCAM1 primary antibodies

  • Description

    Rabbit monoclonal [EPR17010-83] to VCAM1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: NIH/3T3 whole cell lysate; mouse spleen and bone marrow lysates.

  • General notes

    Ab232458 is the carrier-free version of ab181315. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232458 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Immunoprecipitation - Anti-VCAM1 antibody [EPR17010-83] - BSA and Azide free (ab232458)
    Immunoprecipitation - Anti-VCAM1 antibody [EPR17010-83] - BSA and Azide free (ab232458)

    VCAM1 was immunoprecipitated from 1 mg of NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate with ab181315 at 1/50 dilution.

    Western blot was performed from the immunoprecipitate using ab181315 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: NIH/3T3 whole cell lysate 10µg (Input).

    Lane 2: ab181315 IP in NIH/3T3 whole cell lysate.

    Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab181315 in NIH/3T3 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181315).

  • Western blot - Anti-VCAM1 antibody [EPR17010-83] - BSA and Azide free (ab232458)
    Western blot - Anti-VCAM1 antibody [EPR17010-83] - BSA and Azide free (ab232458)
    All lanes : Anti-VCAM1 antibody [EPR17010-83] (ab181315) at 1/1000 dilution

    Lane 1 : NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate
    Lane 2 : Mouse spleen lysate
    Lane 3 : Mouse bone marrow lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lane 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 81 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 3 minutes; Lane 2: 15 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181315).

  • Anti-VCAM1 antibody [EPR17010-83] - BSA and Azide free (ab232458)
    Anti-VCAM1 antibody [EPR17010-83] - BSA and Azide free (ab232458)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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