Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Anti-VCAM1 antibody [EPR5038(2)] (ab174279)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR5038(2)] to VCAM1
Suitable for: WB, IP
Reacts with: Mouse, Human

Product name

Anti-VCAM1 antibody [EPR5038(2)]
See all VCAM1 primary antibodies
Description

Rabbit monoclonal [EPR5038(2)] to VCAM1
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Mouse

WB
Mouse

Human

See all applications and species data
Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human VCAM1 aa 700 to the C-terminus. The exact sequence is proprietary.
Database link: P19320
Positive control
- Human fetal kidney, HuT-78, NIH3T3, HUVEC, bEND.3, Hut-78 and LADMAC lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Mouse
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR5038(2)
Isotype

IgG
Research areas

Western blot - Anti-VCAM1 antibody [EPR5038(2)] (ab174279)

All lanes : Anti-VCAM1 antibody [EPR5038(2)] (ab174279) at 1/1000 dilution

Lane 1 : HUVEC (Human umbilical vein endothelial cell) whole cell lysates
Lane 2 : HUVEC treated with 10 ng/ml TNF-a for 16 h, whole cell lysate
Lane 3 : bEND.3 (Mouse brain endothelioma) whole cell lysates
Lane 4 : bEND.3 treated with 10 µg/ml LPS for 24 h, whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 81 kDa
Observed band size: 100 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Western blot - Anti-VCAM1 antibody [EPR5038(2)] (ab174279)

All lanes : Anti-VCAM1 antibody [EPR5038(2)] (ab174279) at 1/1000 dilution

Lane 1 : Hut-78 (Human Sezary syndrome cutaneous T lymphocyte) whole cell lysates
Lane 2 : SK-OV-3 (Human ovarian cancer epithelial cell) whole cell lysates
Lane 3 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
Lane 4 : J774A.1 (Mouse reticulum cell sarcoma monocyte macrophage) whole cell lysates
Lane 5 : LADMAC (Mouse bone marrow monocyte macrophage) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 81 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?

Exposure time: 15 seconds

Blocking/Diluting buffer and concentration: 5% NFDM/TBST.
Western blot - Anti-VCAM1 antibody [EPR5038(2)] (ab174279)

All lanes : Anti-VCAM1 antibody [EPR5038(2)] (ab174279) at 1/1000 dilution

Lane 1 : Human fetal kidney lysate
Lane 2 : HuT-78 lysate
Lane 3 : NIH3T3 lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 81 kDa
Immunoprecipitation - Anti-VCAM1 antibody [EPR5038(2)] (ab174279)

Immunoprecipitation. ab174279 at 1/1000 labeling VCAM1 immunoprecipitated from NIH3T3 cell lysate using ab174279 at 1/10.
Anti-VCAM1 antibody [EPR5038(2)] (ab174279)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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