Anti-VCAM1 antibody - N-terminal (ab227386)
Key features and details
- Rabbit polyclonal to VCAM1 - N-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-VCAM1 antibody - N-terminal
See all VCAM1 primary antibodies -
Description
Rabbit polyclonal to VCAM1 - N-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Recombinant fragment within Human VCAM1 (N terminal). The exact sequence is proprietary.
Database link: P19320 -
Positive control
- WB: K562 and VCAM1 transfected HEK-293T whole cell lysate. IHC: Human breast cancer tissue.
Properties
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Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VCAM1 antibody - N-terminal (ab227386)
Paraffin-embedded human breast cancer tissue stained for VCAM1 using ab227386 at 1/250 dilution in immunohistochemical analysis.
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Western blot - Anti-VCAM1 antibody - N-terminal (ab227386)Anti-VCAM1 antibody - N-terminal (ab227386) at 1/5000 dilution + K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 81 kDa
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Western blot - Anti-VCAM1 antibody - N-terminal (ab227386)All lanes : Anti-VCAM1 antibody - N-terminal (ab227386) at 1/20000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : VCAM1 transfected HEK-293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 81 kDa
