Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human VCAM1 ELISA Kit (ab223591)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 37 pg/ml
  • Range: 0.14 ng/ml - 9 ng/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human VCAM1 ELISA Kit
    See all VCAM1 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 9 1.4%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 5.23%

  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    37 pg/ml

  • Range

    0.14 ng/ml - 9 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 93 88% - 104%
    Cell culture media 101 92% - 108%
    Hep Plasma 94 89% - 103%
    EDTA Plasma 97 88% - 109%
    Cit plasma 95 92% - 102%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human VCAM1 ELISA Kit (ab223591) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of VCAM1 protein in cit plasma, edta plasma, hep plasma, serum, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human VCAM1 with 37 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Vascular cell adhesion molecule 1 (VCAM1) is a type I transmembrane glycoprotein that facilitates the adhesion of lymphocytes, eosinophils, monocytes and basophils to vascular endothelium. VCAM1 is primarily expressed by endothelial cells, but smooth muscle cells, macrophages, oocytes, Sertoli cells, and activated neurons are all known expressers. VCAM1 expression is stimulated by cytokines, including TNF- a and IL-1, and alternatively spliced forms of the protein are known to occur. VCAM1 may be involved in cardiovascular development, atherosclerosis and rheumatoid arthritis.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • Other - Human VCAM1 ELISA Kit (ab223591)
    Other - Human VCAM1 ELISA Kit (ab223591)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Human VCAM1 Competitor standard curve comparison
    Human VCAM1 Competitor standard curve comparison

    Standard curve comparison between Human VCAM1 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

  • Example of human VCAM1 standard curve [in Sample Diluent NS
    Example of human VCAM1 standard curve [in Sample Diluent NS

    Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native VCAM1 in human serum and plasma, and interpolated concentrations of spiked VCAM1 in cell culture supernatant samples
    Interpolated concentrations of native VCAM1 in human serum and plasma, and interpolated concentrations of spiked VCAM1 in cell culture supernatant samples

    The concentrations of VCAM1 were measured in duplicates, interpolated from the VCAM1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 2.5%, plasma (citrate) 2.5%, plasma (EDTA) 2.5%, plasma (heparin) 2.5%, and RPMI media 20%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VCAM1 concentration was determined to be 1196 ng/mL in neat serum, 1092 ng/mL in neat plasma (citrate), 1275 ng/mL in neat plasma (EDTA), and 976 ng/mL in neat plasma (heparin).

  • Serum from eight individual healthy human female donors was measured in duplicate
    Serum from eight individual healthy human female donors was measured in duplicate

    Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VCAM1 concentration was determined to be 1007 ng/mL with a range of 690 – 1501 ng/mL.

  • Sandwich ELISA - Human VCAM1 ELISA Kit (ab223591)
    Sandwich ELISA - Human VCAM1 ELISA Kit (ab223591)
    To learn more about the advantages of recombinant antibodies see here.

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