Human VCAM1 ELISA Kit (CD106) (ab46118)
Key features and details
- Sensitivity: 0.6 ng/ml
- Range: 1.56 ng/ml - 50 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 2 x 96 tests 1 x 96 tests 10X Standard Diluent Buffer Black 1 x 15ml 1 x 25ml 200X Wash Buffer White 2 x 10ml 1 x 10ml Biotinylated Antibody Diluent Red 1 x 13ml 1 x 7.5ml Biotinylated anti-VCAM1 Red 2 x 400µl 1 x 400µl Chromogen TMB Substrate Solution 1 x 24ml 1 x 11ml Control Silver 4 vials 2 vials HRP Diluent Red 1 x 12ml 1 x 23ml Stop Reagent Black 2 x 11ml 1 x 11ml Streptavidin-HRP 4 x 5µl 2 x 5µl VCAM1 Microplate (12 x 8 well strips) 2 units 1 unit VCAM1 Standard (lyophilized) Yellow 4 vials 2 vials -
Research areas
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Function
Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. -
Tissue specificity
Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue. -
Sequence similarities
Contains 7 Ig-like C2-type (immunoglobulin-like) domains. -
Domain
Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion. -
Post-translational
modificationsSialoglycoprotein. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- CD106
- CD106 Antigen
- INCAM 100
see all -
Database links
- Entrez Gene: 7412 Human
- GenBank: NP_001069.1 Human
- GenBank: NP_001186763.1 Human
- Omim: 192225 Human
- SwissProt: P19320 Human
- Unigene: 109225 Human
Images
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Typical Standard Curve
Representative Standard Curve using ab46118.
