Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Bag3 antibody [EPR20207-58-4] (ab246225)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20207-58-4] to Bag3
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Bag3 antibody [EPR20207-58-4]
    See all Bag3 primary antibodies

  • Description

    Rabbit monoclonal [EPR20207-58-4] to Bag3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human skeletal muscle lysate; HeLa whole cell lysate. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Bag3 antibody [EPR20207-58-4] (ab246225)
    Immunocytochemistry/ Immunofluorescence - Anti-Bag3 antibody [EPR20207-58-4] (ab246225)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) and HL-60 (human promyelocytic leukemia cell line) cells labeling Bag3 with ab246225 at 1/70 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and weakly nuclear staining in HeLa cell line. Negative control: HL-60 (PMID: 17974966).

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    Control: PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Western blot - Anti-Bag3 antibody [EPR20207-58-4] (ab246225)
    Western blot - Anti-Bag3 antibody [EPR20207-58-4] (ab246225)
    All lanes : Anti-Bag3 antibody [EPR20207-58-4] (ab246225) at 1/1000 dilution

    Lane 1 : Human skeletal muscle lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : HL-60 (human promyelocytic leukemia cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 62 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?



    Exposure time : Lane 1: 30 seconds; Lane 2: 170 seconds; Lane 3: 180 seconds.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Either Goat Anti-Rabbit IgG H&L (HRP) (ab97051) or VeriBlot for IP Detection Reagent (HRP) (ab131366), were used for detection.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 17974966).

    Negative control: HL-60 (PMID: 17974966).

  • Flow Cytometry - Anti-Bag3 antibody [EPR20207-58-4] (ab246225)
    Flow Cytometry - Anti-Bag3 antibody [EPR20207-58-4] (ab246225)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HL-60  (human promyelocytic leukemia cell line) (Left) and HeLa (human epithelial cell line from cervix adenocarcinoma) (Right) cells labeling Bag3 with ab246225 at 1/70 dilution (red) compared witha Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

    Negative control: HL-60 (PMID: 17974966).

  • Anti-Bag3 antibody [EPR20207-58-4] (ab246225)
    Anti-Bag3 antibody [EPR20207-58-4] (ab246225)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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