Anti-hHR23A antibody (ab240610)
Key features and details
- Rabbit polyclonal to hHR23A
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-hHR23A antibody
See all hHR23A primary antibodies -
Description
Rabbit polyclonal to hHR23A -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human hHR23A aa 200-250. The exact sequence is proprietary.
Database link: P54725 -
Positive control
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysate. IP: hHR23A IP in HeLa whole cell lysate. IHC-P: Human lung carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-hHR23A antibody (ab240610)All lanes : Anti-hHR23A antibody (ab240610) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Exposure time: 10 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hHR23A antibody (ab240610)Formalin-fixed, paraffin-embedded human lung cancer carcinoma tissue stained for hHR23A using ab240610 at 1/1000 dilution in immunohistochemical analysis. DAB staining.
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Immunoprecipitation - Anti-hHR23A antibody (ab240610)hHR23A was immunoprecipitated from 1 mg (20% of IP loaded) HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate using ab240610 at 3 μg/mL lysate. Western blot was performed on the immunoprecipitate using ab240610 at 0.4 μg/mL.
Lane 1: ab240610 IP in HeLa whole cell lysate.
Lane 2: Control IgG (-).Detection: Chemiluminescence with exposure times of 10 seconds
