Antibodies, Proteins, Kits and Reagents for Life Science

274 732 ₸ Product size

100 µg 274 732 ₸

Order now and get it on Wednesday March 24, 2021

Anti-Mitochondria antibody [AE-1] (ab215892)

Key features and details

Mouse monoclonal [AE-1] to Mitochondria
Suitable for: ICC, WB, IHC-P
Reacts with: Human
Isotype: IgG1

Product name

Anti-Mitochondria antibody [AE-1]
See all Mitochondria primary antibodies
Description

Mouse monoclonal [AE-1] to Mitochondria
Host species

Mouse
Tested applications

Suitable for: ICC, WB, IHC-Pmore details
Species reactivity

Reacts with: Human
Does not react with: Mouse, Rat
Immunogen

Tissue, cells or virus corresponding to Human Mitochondria. Semi-purified mitochondrial preparation
Positive control
- IHC: Human tonsil and pancreas tissues. ICC: Human HeLa cells WB: Human HeLa cells
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A/G purified
Purification notes

ab215892 is purified from Bioreactor Concentrate by Protein A/G.
Clonality

Monoclonal
Clone number

AE-1
Isotype

IgG1
Light chain type

kappa
Research areas

Western blot - Anti-Mitochondria antibody [AE-1] (ab215892)

Anti-Mitochondria antibody [AE-1] (ab215892) at 0.5 µg/ml + HeLa cells (human epithelial cell line from cervix adenocarcinoma)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitochondria antibody [AE-1] (ab215892)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling mitochondria with ab215892 at 2µg/ml dilution. Heat mediated antigen retrieval performed with tris/EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitochondria antibody [AE-1] (ab215892)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling mitochondria with ab215892 at 2µg/ml dilution. Heat mediated antigen retrieval performed with tris/EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunocytochemistry - Anti-Mitochondria antibody [AE-1] (ab215892)

Immunocytochemistry confocal immunoflourescence of HeLa cells (human epithelial cell line from cervix adenocarcinoma) stained with ab215892 2µg/ml in green, and nuclear counter staining in RedDot red.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitochondria antibody [AE-1] (ab215892)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling Mitochondria with ab215892 at 1µg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitochondria antibody [AE-1] (ab215892)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue labeling Mitochondria with ab215892 at 1µg/ml.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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