Anti-Mitochondria antibody [AE-1] (ab215892)
![Anti-Mitochondria antibody [AE-1] (ab215892)](https://www.abcam.com/ps/products/215/ab215892/Images/ab215892-456596-anti-mitochondria-antibody-western-blot-hela-cell-human.jpg "Anti-Mitochondria antibody [AE-1] (ab215892)")
Key features and details
- Mouse monoclonal [AE-1] to Mitochondria
- Suitable for: ICC, WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Mitochondria antibody [AE-1]
See all Mitochondria primary antibodies -
Description
Mouse monoclonal [AE-1] to Mitochondria -
Host species
Mouse -
Tested applications
Suitable for: ICC, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Tissue, cells or virus corresponding to Human Mitochondria. Semi-purified mitochondrial preparation
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Positive control
- IHC: Human tonsil and pancreas tissues. ICC: Human HeLa cells WB: Human HeLa cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
ab215892 is purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
AE-1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-Mitochondria antibody [AE-1] (ab215892)Anti-Mitochondria antibody [AE-1] (ab215892) at 0.5 µg/ml + HeLa cells (human epithelial cell line from cervix adenocarcinoma)
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitochondria antibody [AE-1] (ab215892)](https://www.abcam.com/ps/products/215/ab215892/Images/ab215892-456595-anti-mitochondria-antibody-immunohistochemistry-tonsil-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitochondria antibody [AE-1] (ab215892)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling mitochondria with ab215892 at 2µg/ml dilution. Heat mediated antigen retrieval performed with tris/EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitochondria antibody [AE-1] (ab215892)](https://www.abcam.com/ps/products/215/ab215892/Images/ab215892-456594-anti-mitochondria-antibody-immunohistochemistry-pancreas-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitochondria antibody [AE-1] (ab215892)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling mitochondria with ab215892 at 2µg/ml dilution. Heat mediated antigen retrieval performed with tris/EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Immunocytochemistry - Anti-Mitochondria antibody [AE-1] (ab215892)](https://www.abcam.com/ps/products/215/ab215892/Images/ab215892-456593-anti-mitochondria-antibody-immunocytochemistry-hela-human.jpg)
Immunocytochemistry - Anti-Mitochondria antibody [AE-1] (ab215892)Immunocytochemistry confocal immunoflourescence of HeLa cells (human epithelial cell line from cervix adenocarcinoma) stained with ab215892 2µg/ml in green, and nuclear counter staining in RedDot red.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitochondria antibody [AE-1] (ab215892)](https://www.abcam.com/ps/products/215/ab215892/Images/ab215892-269193-anti-mitochondria-antibody-ae-1-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitochondria antibody [AE-1] (ab215892)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling Mitochondria with ab215892 at 1µg/ml.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitochondria antibody [AE-1] (ab215892)](https://www.abcam.com/ps/products/215/ab215892/Images/ab215892-269194-anti-mitochondria-antibody-ae-1-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitochondria antibody [AE-1] (ab215892)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue labeling Mitochondria with ab215892 at 1µg/ml.
