Human Transferrin Receptor ELISA Kit (CD71) (ab272469)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 18 pg/ml
- Range: 53.13 pg/ml - 3400 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, Cit plasma, EDTA Plasma, Hep Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human Transferrin Receptor ELISA Kit (CD71)
See all Transferrin Receptor kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 7 3.4% Inter-assay Sample n Mean SD CV% Serum 3 3.9% -
Sample type
Cell culture supernatant, Saliva, Urine, Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
18 pg/ml -
Range
53.13 pg/ml - 3400 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 94 90% - 101% Saliva 75 73% - 76% Urine 89 88% - 90% Serum 86 82% - 94% Hep Plasma 106 103% - 108% EDTA Plasma 93 82% - 100% Cit plasma 110 107% - 112% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Transferrin Receptor ELISA kit (ab272469) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Transferrin Receptor protein in serum, plasma, urine, saliva, cell culture supernatant and cell and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Transferrin Receptor with 18 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
Notes
Transferrin receptor, also known as CD71 TfR1, is a 760 amino acid, transmembrane glycoprotein of plasma membrane composed of two disulfide-linked monomers encoded by the gene TFRC. Transferrin receptor is necessary to regulate iron transport into cells from transferrin by receptor mediated endocytosis. The soluble form of transferrin receptor is due to the proteolysis of Arg-100 in the extracellular domain. Measurements of soluble transferrin receptor may be an indication of iron deficiency due to chronic diseases
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Transferrin Receptor (CD71) Capture Antibody 1 x 600µl 10X Human Transferrin Receptor (CD71) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human Transferrin Receptor (CD71) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 2 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
-
Function
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
(Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus. -
Involvement in disease
Immunodeficiency 46 -
Sequence similarities
Belongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain. -
Post-translational
modificationsN- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation. -
Cellular localization
Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
-
Alternative names
- CD 71
- CD71
- CD71 antigen
see all -
Database links
- Entrez Gene: 7037 Human
- Omim: 190010 Human
- SwissProt: P02786 Human
- Unigene: 529618 Human
Images
-
SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Human Transferrin Receptor competitor curve comparisonStandard Curve comparison between Human Transferrin Receptor SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
-
Human Transferrin Receptor standard curve comparisonStandard Curve comparison between the original Human Transferrin Receptor SimpleStep ELISA kit (ab217780) and current Human Transferrin Receptor SimpleStep ELISA kit (ab272469).
-
Example of human Transferrin Receptor standard curve in Sample Diluent NS.The Transferrin Receptor standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
-
Interpolated concentrations of native Transferrin Receptor in human serum and plasma samples.The concentrations of Transferrin Receptor were measured in duplicates, interpolated from the Transferrin Receptor standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:1,000, plasma (citrate) 1:1,000, plasma (EDTA) 1:500 and plasma (heparin) 1:1,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Transferrin Receptor concentration was determined to be 2,122 ng/mL in neat serum, 2,234 ng/mL in neat plasma (citrate), 1,714 ng/mL in neat plasma (EDTA) and 1,990 ng/mL in neat plasma (heparin).
-
Interpolated concentrations of native Transferrin Receptor in human urine, saliva and A549 cell culture supernatant samples.The concentrations of Transferrin Receptor were measured in duplicates, interpolated from the Transferrin Receptor standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 25%, saliva 12.5%, and A549 supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Transferrin Receptor concentration was determined to be 829 pg/mL in neat urine, 3,217 pg/mL in neat saliva and 1,755 pg/mL in neat A549 supernatant.
-
Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Transferrin Receptor concentration was determined to be 3,747 ng/mL with a range of 2,378 – 6,239 ng/mL.
-
Interpolated concentrations of native Transferrin Receptor in human A549 cell based on a 25 µg/mL extract load.The concentrations of Transferrin Receptor were measured in duplicate and interpolated from the Transferrin Receptor standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Transferrin Receptor concentration was determined to be 2,157 pg/mL in A549 cell extract.
-
Sandwich ELISA - Human Transferrin Receptor ELISA Kit (CD71) (ab272469)To learn more about the advantages of recombinant antibodies see here.
