Anti-alpha 1 Antitrypsin antibody (ab9373)
Key features and details
- Rabbit polyclonal to alpha 1 Antitrypsin
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-alpha 1 Antitrypsin antibody
See all alpha 1 Antitrypsin primary antibodies -
Description
Rabbit polyclonal to alpha 1 Antitrypsin -
Host species
Rabbit -
Specificity
The antibody is specific against human alpha-1-antitrypsin. -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mammals
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Immunogen
Alpha-1-antitrypsin isolated from human serum.
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Positive control
- IHC-P: Human tonsil tissue. ICC/IF: HepG2 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Light chain type
unknown -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody (ab9373)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling alpha 1 Antitrypsin with ab9373.
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Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Antitrypsin antibody (ab9373)ICC/IF image of ab9373 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9373, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody (ab9373)ab9373 staining alpha 1 Antitrypsin in formalin-fixed, paraffin-embedded Human tonsil tissue by Immunohistochemistry.
