Anti-MPP1 antibody [EPR5865] (ab108528)
![Anti-MPP1 antibody [EPR5865] (ab108528)](https://www.abcam.com/ps/products/108/ab108528/Images/ab108528-410196-anti-mpp1-antibody-epr5865-western-blot-human-knockout-lysate.jpg "Anti-MPP1 antibody [EPR5865] (ab108528)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5865] to MPP1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-MPP1 antibody [EPR5865]
See all MPP1 primary antibodies -
Description
Rabbit monoclonal [EPR5865] to MPP1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: K562, 293T, and Jurkat cell lysates
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5865 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MPP1 antibody [EPR5865] (ab108528)All lanes : Anti-MPP1 antibody [EPR5865] (ab108528) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : MPP1 knockout HEK293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : K-562 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDaLanes 1-4: Merged signal (red and green). Green - ab108528 observed at 52 kDa. Red - loading control ab8245 observed at 36 kDa.
ab108528 Anti-MPP1 antibody [EPR5865] was shown to specifically react with MPP1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab267248 (knockout cell lysate ab258051) was used. Wild-type and MPP1 knockout samples were subjected to SDS-PAGE. ab108528 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-MPP1 antibody [EPR5865] (ab108528)](https://www.abcam.com/ps/products/108/ab108528/Images/ab108528-121249-anti-mpp1-antibody-epr5865-western-blot.jpg)
Western blot - Anti-MPP1 antibody [EPR5865] (ab108528)All lanes : Anti-MPP1 antibody [EPR5865] (ab108528) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 52 kDa
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![Anti-MPP1 antibody [EPR5865] (ab108528)](https://www.abcam.com/ps/products/108/ab108528/Images/ab108528-3-benefits-of-recombinant-antibodies.png)
Anti-MPP1 antibody [EPR5865] (ab108528)
