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Cardiovascular Blood Serum Proteins

Anti-alpha 1 Antitrypsin antibody [EPR9090] (ab166610)

Price and availability

328 339 ₸

Availability

Order now and get it on Thursday March 04, 2021

Anti-alpha 1 Antitrypsin antibody [EPR9090] (ab166610)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9090] to alpha 1 Antitrypsin
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

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Overview

  • Product name

    Anti-alpha 1 Antitrypsin antibody [EPR9090]
    See all alpha 1 Antitrypsin primary antibodies
  • Description

    Rabbit monoclonal [EPR9090] to alpha 1 Antitrypsin
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Human alpha 1 Antitrypsin.

  • Positive control

    • WB: Human serum and fetal liver lysates. ICC/IF: HepG2 cells.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR9090
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Serum Proteins
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Antitrypsin antibody [EPR9090] (ab166610)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Antitrypsin antibody [EPR9090] (ab166610)

    ab166610 stained HepG2 cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab166610 at 1/100 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Western blot - Anti-alpha 1 Antitrypsin antibody [EPR9090] (ab166610)
    Western blot - Anti-alpha 1 Antitrypsin antibody [EPR9090] (ab166610)
    All lanes : Anti-alpha 1 Antitrypsin antibody [EPR9090] (ab166610) at 1/1000 dilution

    Lane 1 : Human serum lysate
    Lane 2 : Fetal liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 46 kDa
    Observed band size: 52 kDa
    why is the actual band size different from the predicted?

  • Anti-alpha 1 Antitrypsin antibody [EPR9090] (ab166610)
    Anti-alpha 1 Antitrypsin antibody [EPR9090] (ab166610)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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