Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Desmoplakin antibody [EPR4383(2)] (ab109445)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4383(2)] to Desmoplakin
  • Suitable for: Flow Cyt, WB, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-Desmoplakin antibody [EPR4383(2)]
    See all Desmoplakin primary antibodies

  • Description

    Rabbit monoclonal [EPR4383(2)] to Desmoplakin

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A431, HACAT and BXPC-3 cell lysates. ICC/IF: A431 cells. Flow Cyt: A431 cells.

  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Desmoplakin antibody [EPR4383(2)] (ab109445)
    Western blot - Anti-Desmoplakin antibody [EPR4383(2)] (ab109445)
    All lanes : Anti-Desmoplakin antibody [EPR4383(2)] (ab109445) at 1/1000 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : HACAT cell lysate
    Lane 3 : BXPC-3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 332 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Desmoplakin antibody [EPR4383(2)] (ab109445)
    Immunocytochemistry/ Immunofluorescence - Anti-Desmoplakin antibody [EPR4383(2)] (ab109445)

    Immunocytochemistry/Immunofluorescence analysis of A431 cells labelling Desmoplakin with ab109445 at 1/1000. Cells were fixed with 100% methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
    Control: PBS only.
    Nuclear counter stain: DAPI.

  • Flow Cytometry - Anti-Desmoplakin antibody [EPR4383(2)] (ab109445)
    Flow Cytometry - Anti-Desmoplakin antibody [EPR4383(2)] (ab109445)

    Flow cytometry analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling Desmoplakin (red) with ab109445 at a 1/250 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Anti-Desmoplakin antibody [EPR4383(2)] (ab109445)
    Anti-Desmoplakin antibody [EPR4383(2)] (ab109445)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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