Anti-alpha 1 Antitrypsin antibody [11G2] (ab230695)
![Anti-alpha 1 Antitrypsin antibody [11G2] (ab230695)](https://www.abcam.com/ps/products/230/ab230695/Images/ab230695-299009-ab2306952.jpg "Anti-alpha 1 Antitrypsin antibody [11G2] (ab230695)")
Key features and details
- Mouse monoclonal [11G2] to alpha 1 Antitrypsin
- Suitable for: IHC-P, WB, IP
- Reacts with: Dog, Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-alpha 1 Antitrypsin antibody [11G2]
See all alpha 1 Antitrypsin primary antibodies -
Description
Mouse monoclonal [11G2] to alpha 1 Antitrypsin -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Dog, Human, African green monkey -
Immunogen
Recombinant full length protein corresponding to Human alpha 1 Antitrypsin. (Expressed in HEK-293T cells).
Database link: P01009 -
Positive control
- IHC-P: Human liver tissue. WB: HEK-293T cell lysate transfected with pCMV6-ENTRY alpha 1 Antitrypsin; HepG2, HeLa, HT-29, COS-7, Jurkat, MDCK and MCF7 cell extracts.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from TCS by Protein A/G. -
Clonality
Monoclonal -
Clone number
11G2 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-alpha 1 Antitrypsin antibody [11G2] (ab230695)All lanes : Anti-alpha 1 Antitrypsin antibody [11G2] (ab230695) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY alpha 1 Antitrypsin
Lysates/proteins at 5 µg per lane.
Predicted band size: 46 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody [11G2] (ab230695)](https://www.abcam.com/ps/products/230/ab230695/Images/ab230695-299010-ab2306951.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody [11G2] (ab230695)Formalin-fixed, paraffin-embedded human liver tissue stained for alpha 1 Antitrypsin using ab230695 at 10 µg/ml in immunohistochemical analysis.
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![Western blot - Anti-alpha 1 Antitrypsin antibody [11G2] (ab230695)](https://www.abcam.com/ps/products/230/ab230695/Images/ab230695-299008-ab2306953.jpg)
Western blot - Anti-alpha 1 Antitrypsin antibody [11G2] (ab230695)All lanes : Anti-alpha 1 Antitrypsin antibody [11G2] (ab230695) at 1/1000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 3 : HT-29 (human colorectal adenocarcinoma cell line) cell extract
Lane 4 : A549 (human lung carcinoma cell line) cell extract
Lane 5 : COS-7 (african green monkey kidney fibroblast-like cell line) cell extract
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) cell extract
Lane 7 : MDCK (canine kidney cell line) cell extract
Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell extract
Lane 9 : MCF7 (human breast adenocarcinoma cell line) cell extract
Lysates/proteins at 35 µg per lane.
Predicted band size: 46 kDa
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![Immunoprecipitation - Anti-alpha 1 Antitrypsin antibody [11G2] (ab230695)](https://www.abcam.com/ps/products/230/ab230695/Images/ab230695-299006-ab230695IP.jpg)
Immunoprecipitation - Anti-alpha 1 Antitrypsin antibody [11G2] (ab230695)alpha 1 Antitrypsin was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) DDK tagged alpha 1 Antitrypsin antibody overexpression lysate with 2 µg ab230695 and 20 µl (0.1 mg) of goat anti-mouse conjugated magnetic beads. Western blot was performed from the immunoprecipitate using rabbit anti-DDK polyclonal antibody.
Lane 1: Negative control: IP without adding anti-alpha 1 Antitrypsin antibody in HEK-293T DDK tagged alpha 1 Antitrypsin antibody overexpression lysate.
Lane 2: ab230695 IP in -293T DDK tagged alpha 1 Antitrypsin antibody overexpression lysate.
