Astana Biomed Group, an authorized Abcam distributor in Central Asia

Rat Retinol Binding Protein 4 ELISA Kit (ab203362)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 3.07 pg/ml
  • Range: 13.3 pg/ml - 850 pg/ml
  • Sample type: Cell culture supernatant, Plasma, Serum, Urine
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Rat

Overview

  • Product name

    Rat Retinol Binding Protein 4 ELISA Kit
    See all RBP4 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 5 3.3%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 2.7%

  • Sample type

    Cell culture supernatant, Urine, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    3.07 pg/ml

  • Range

    13.3 pg/ml - 850 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 116 109% - 126%
    Urine 104 103% - 105%
    Serum 113 107% - 116%
    Cit plasma 100 91% - 106%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Rat
    Does not react with: Sheep, Goat, Cow, Pig

  • Product overview

    Rat Retinol Binding Protein 4 ELISA Kit (ab203362) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Retinol Binding Protein 4 protein in cell culture supernatant, serum, urine, and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat Retinol Binding Protein 4 with 3.07 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Retinol-Binding Protein 4 (RBP4) is a small, highly conserved member of the lipocalin superfamily involved in the transportation of retinol (Vitamin A) in blood. Synthesized in hepatocytes and adipocytes, RBP4 is also an adipokine involved in insulin resistance in the AG4KO mouse model. Elevated serum levels of RBP4 are seen in obesity and Type 2 diabetes.

  • Platform

    Microplate (12 x 8 well strips)

Properties

Images

  • Other - Rat Retinol Binding Protein 4 ELISA Kit (ab203362)
    Other - Rat Retinol Binding Protein 4 ELISA Kit (ab203362)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of RBP4 standard curve prepared in Sample Diluent NS.
    Example of RBP4 standard curve prepared in Sample Diluent NS.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of RBP4 in rat plasma (citrate) and serum.
    Interpolated concentrations of RBP4 in rat plasma (citrate) and serum.

    The concentrations of RBP4 were measured in duplicate and interpolated from the RBP4 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Sandwich ELISA - Rat Retinol Binding Protein 4 ELISA Kit (ab203362)
    Sandwich ELISA - Rat Retinol Binding Protein 4 ELISA Kit (ab203362)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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