Anti-MMP13 antibody (ab39012)
Key features and details
- Rabbit polyclonal to MMP13
- Suitable for: IHC-P, WB, IHC-Fr, ICC/IF, Flow Cyt, Sandwich ELISA
- Reacts with: Mouse, Rat, Dog, Human
- Isotype: IgG
Overview
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Product name
Anti-MMP13 antibody
See all MMP13 primary antibodies -
Description
Rabbit polyclonal to MMP13 -
Host species
Rabbit -
Specificity
ab39012 recognizes the latent proenzyme, at 60 Kd, as well as the active form at 48 Kd, and intermediate activation forms. It does not cross react with the other MMP family members. ab39012 recognizes the Hinge region of MMP13. -
Tested applications
Suitable for: IHC-P, WB, IHC-Fr, ICC/IF, Flow Cyt, Sandwich ELISAmore details -
Species reactivity
Reacts with: Mouse, Rat, Dog, Human -
Immunogen
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody has been peptide-affinity purified. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MMP13 antibody (ab39012)All lanes : Anti-MMP13 antibody (ab39012) at 1/3000 dilution
Lane 1 : cell media from human chondrosarcoma (untreated).
Lane 2 : cell media from human chondrosarcoma (IL1 beta treated).
Predicted band size: 54 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
The loading amount is 15 ul/lane, and the condrosarcoma media was concentrated 40x. -
Sandwich ELISA - Anti-MMP13 antibody (ab39012)Standard curve for MMP13; dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [181-15A12] to MMP13 (ab77949) at 1µg/ml and Detector Antibody Rabbit polyclonal to MMP13 - Hinge region (ab39012) at 0.5µg/ml.
