Anti-MMP13 antibody [EPR21778] (ab219620)
![Anti-MMP13 antibody [EPR21778] (ab219620)](https://www.abcam.com/ps/products/219/ab219620/Images/ab219620-314817-anti-mmp13-antibody-epr21778-immunohistochemistry.jpg "Anti-MMP13 antibody [EPR21778] (ab219620)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21778] to MMP13
- Suitable for: IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-MMP13 antibody [EPR21778]
See all MMP13 primary antibodies -
Description
Rabbit monoclonal [EPR21778] to MMP13 -
Host species
Rabbit -
Specificity
ab219620 showed specific staining in cortical bone, but that no staining was observed in the breast cancer tissue tested. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseRatHuman
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human tibia tissue; Mouse and rat femur tissues. Recommend to test on the cortical bone as a positive control (PMID: 22549931). Mmp13 is expressed in mature osteoblasts (PMID: 17987127), other tissue are proved to be negative under normal condition (PMID: 17179173).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21778 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [EPR21778] (ab219620)
Immunohistochemical analysis of paraffin-embedded rat femur tissue labeling MMP13 with ab219620 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on cortical bone of rat femur (PMID: 22549931) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [EPR21778] (ab219620)](https://www.abcam.com/ps/products/219/ab219620/Images/ab219620-314816-anti-mmp13-antibody-epr21778-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [EPR21778] (ab219620)Immunohistochemical analysis of paraffin-embedded mouse femur tissue labeling MMP13 with ab219620 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on cortical bone of mouse femur (PMID: 22549931) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [EPR21778] (ab219620)](https://www.abcam.com/ps/products/219/ab219620/Images/ab219620-314815-anti-mmp13-antibody-epr21778-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [EPR21778] (ab219620)Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling MMP13 with ab219620 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Counter stained with hematoxylin.
Negative control: No staining on human skeletal muscle (PMID: 9056642) is observed.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [EPR21778] (ab219620)](https://www.abcam.com/ps/products/219/ab219620/Images/ab219620-314814-anti-mmp13-antibody-epr21778-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [EPR21778] (ab219620)Immunohistochemical analysis of paraffin-embedded human tibia tissue labeling MMP13 with ab219620 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on cortical bone of human tibia (PMID: 22549931) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Anti-MMP13 antibody [EPR21778] (ab219620)](https://www.abcam.com/ps/products/219/ab219620/Images/ab219620-5-benefits-of-recombinant-antibodies.png)
Anti-MMP13 antibody [EPR21778] (ab219620)
