Anti-MMP13 antibody (ab84594)
Key features and details
- Rabbit polyclonal to MMP13
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MMP13 antibody
See all MMP13 primary antibodies -
Description
Rabbit polyclonal to MMP13 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human MMP13 aa 250-350 conjugated to keyhole limpet haemocyanin.
(Peptide available asab93499)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab84594 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatRabbitHorseApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 54 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 54 kDa).Target
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Function
Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process. -
Tissue specificity
Seems to be specific to breast carcinomas. -
Involvement in disease
Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia Missouri type (SEMD-MO) [MIM:602111]. A bone disease characterized by moderate to severe metaphyseal changes, mild epiphyseal involvement, rhizomelic shortening of the lower limbs with bowing of the femora and/or tibiae, coxa vara, genu varum and pear-shaped vertebrae in childhood. Epimetaphyseal changes improve with age.
Defects in MMP13 are the cause of metaphyseal anadysplasia type 1 (MANDP1) [MIM:602111]. Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia. -
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 4322 Human
- Entrez Gene: 17386 Mouse
- Entrez Gene: 171052 Rat
- Omim: 600108 Human
- SwissProt: O18927 Horse
- SwissProt: P45452 Human
- SwissProt: P33435 Mouse
- SwissProt: O62806 Rabbit
see all -
Alternative names
- CLG 3 antibody
- CLG3 antibody
- Collagenase 3 antibody
see all
Images
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Western blot - Anti-MMP13 antibody (ab84594)All lanes : Anti-MMP13 antibody (ab84594) at 1 µg/ml
Lane 1 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
The bands observed at 46 kDa could potentially be a cleaved form of Human Collagenase 3 precursor. The first 19 amino acids in the sequence act as a signal peptide and the following 84 (A.A 20-103) act as a activation peptide. Once cleaved this gives the target protein an expected weight of 43 kDa.
Datasheets and documents
References (13)
ab84594 has been referenced in 13 publications.
- Zhu H et al. CircGCN1L1 promotes synoviocyte proliferation and chondrocyte apoptosis by targeting miR-330-3p and TNF-a in TMJ osteoarthritis. Cell Death Dis 11:284 (2020). PubMed: 32332704
- Li H et al. Involvement of the G-Protein-Coupled Receptor 4 in the Increased Expression of RANK/RANKL/OPG System and Neurotrophins by Nucleus Pulposus Cells under the Degenerated Intervertebral Disc-Like Acidic Microenvironment. Biomed Res Int 2020:1328436 (2020). PubMed: 32566653
- Tabeian H et al. IL-1ß Damages Fibrocartilage and Upregulates MMP-13 Expression in Fibrochondrocytes in the Condyle of the Temporomandibular Joint. Int J Mol Sci 20:N/A (2019). PubMed: 31067826
- Arias C et al. Propolis Reduces the Expression of Autophagy-Related Proteins in Chondrocytes under Interleukin-1ß Stimulus. Int J Mol Sci 20:N/A (2019). PubMed: 31374866
- Liu X et al. MiR-146b accelerates osteoarthritis progression by targeting alpha-2-macroglobulin. Aging (Albany NY) 11:6014-6028 (2019). PubMed: 31422941
Images
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Western blot - Anti-MMP13 antibody (ab84594)All lanes : Anti-MMP13 antibody (ab84594) at 1 µg/ml
Lane 1 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
The bands observed at 46 kDa could potentially be a cleaved form of Human Collagenase 3 precursor. The first 19 amino acids in the sequence act as a signal peptide and the following 84 (A.A 20-103) act as a activation peptide. Once cleaved this gives the target protein an expected weight of 43 kDa.
