Bovine SAA ELISA Kit (ab274407)
Key features and details
- Range: 26.34 ng/ml - 300 ng/ml
- Sample type: Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Cow
Overview
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Product name
Bovine SAA ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Urine, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Range
26.34 ng/ml - 300 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum > 85 % - % -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Cow -
Product overview
The Bovine SAA ELISA kit (ab274407) is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring SAA in bovine biological samples.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 50ml 5X Diluent Buffer Concentrate 1 x 50ml Antibody coated plate 1 unit Bovine SAA Calibrator (Lyophilized) 1 vial 100X HRP-conjugated Bovine SAA antibody 1 vial Stop Solution 1 x 12ml Chromogen Substrate Solution 1 x 12ml -
Research areas
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Relevance
The serum amyloid A family comprises a number of differentially expressed apolipoproteins, acute-phase SAA1 and SAA2, the former being the major component in plasma and constitutive SAAs. Although the liver is the primary site of synthesis of both SAA types extrahepatic production has been reported. The in vivo concentrations increase by as much as 1000 fold during inflammation. Several studies have expressed its importance in the diagnosis and monitoring of various diseases. Pathological SAA values are often detected in association with normal CRP concentrations; SAA rises earlier and more sharply than CRP. Recently, a broader view of SAA expression and function has been emerging. Expression studies show production of SAA proteins in histologically normal, atherosclerotic, Alzheimer, inflammatory, and tumour tissues. SAA has been found to have binding sites for high density lipoproteins, calcium, laminin, and heparin/heparin sulphate. Also adhesion motifs were identified and new functions affecting cell adhesion, migration, proliferation, and aggregation were discovered. These findings emphasize the importance of SAA in various physiological and pathological processes including inflammation, atherosclerosis, thrombosis, AA-amyloidosis, rheumatoid arthritis, and neoplasia. SAA has also a number of immunomodulatory roles, it can induce chemotaxis and adhesion molecule expression, has cytokine-like properties and can promote the upregulation of metalloproteinases. It enhances the binding of high density lipoprotein to macrophages and thus helps in the delivery of lipids to sites of injury for use in tissue repair, it is thus thought to be an integral part of the disease process. -
Cellular localization
Secreted -
Alternative names
- Amyloid fibril protein AA
- Amyloid protein A
- MGC111216
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