Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IRAK-1 antibody [EPR18630] - BSA and Azide free (ab250249)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18630] to IRAK-1 - BSA and Azide free
  • Suitable for: ICC, IP, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-IRAK-1 antibody [EPR18630] - BSA and Azide free
    See all IRAK-1 primary antibodies

  • Description

    Rabbit monoclonal [EPR18630] to IRAK-1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IP, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250249 is the carrier-free version of ab180747. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250249 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-IRAK-1 antibody [EPR18630] - BSA and Azide free (ab250249)
    Western blot - Anti-IRAK-1 antibody [EPR18630] - BSA and Azide free (ab250249)

    This data was developed using ab180747, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)

    Lane 2: IRAK-1 knockout HAP1 whole cell lysate (20 µg)

    Lane 3: HeLa whole cell lysate (20 µg)

    Lane 4: Hek293 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab180747 observed at 85 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab180747 was shown to specifically react with IRAK-1 when IRAK-1 knockout samples were used. Wild-type and IRAK-1 knockout samples were subjected to SDS-PAGE. ab180747 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti-Rabbit and 680CW Goat anti-Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-IRAK-1 antibody [EPR18630] - BSA and Azide free (ab250249)
    Western blot - Anti-IRAK-1 antibody [EPR18630] - BSA and Azide free (ab250249)
    All lanes : Anti-IRAK-1 antibody [EPR18630] (ab180747) at 1/1000 dilution

    Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 3 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 77 kDa
    Observed band size: 77 kDa



    This data was developed using ab180747, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 10 seconds; Lane 2: 3 minutes; Lane 3: 30 seconds; Lane 4: 1 minute.

    There are two splice variants for IRAK-1, IRAK-1b and IRAK-1c, respectively. The product recognizes full length IRAK-1 (80kDa).

  • Immunocytochemistry - Anti-IRAK-1 antibody [EPR18630] - BSA and Azide free (ab250249)
    Immunocytochemistry - Anti-IRAK-1 antibody [EPR18630] - BSA and Azide free (ab250249)
    This data was developed using ab180747, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling IRAK-1 with ab180747 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red). The negative controls are as follows:
    -ve control 1: ab180747 at 1/250 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
    -ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
  • Immunocytochemistry - Anti-IRAK-1 antibody [EPR18630] - BSA and Azide free (ab250249)
    Immunocytochemistry - Anti-IRAK-1 antibody [EPR18630] - BSA and Azide free (ab250249)
    This data was developed using ab180747, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized K562 (Human chronic myelogenous leukemia cell line from bone marrow) cells labeling IRAK-1 with ab180747 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on K562 cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red). The negative controls are as follows:
    -ve control 1: ab180747 at 1/250 dilution, followed by followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
    -ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
  • Immunoprecipitation - Anti-IRAK-1 antibody [EPR18630] - BSA and Azide free (ab250249)
    Immunoprecipitation - Anti-IRAK-1 antibody [EPR18630] - BSA and Azide free (ab250249)
    This data was developed using ab180747, the same antibody clone in a different buffer formulation.IRAK-1 was immunoprecipitated from 0.35 mg of HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate with ab180747 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab180747 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution. Lane 1: HEK-293 whole cell lysate 10µg (Input). Lane 2: ab180747 IP in HEK-293 whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab180747 in HEK-293 whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 30 seconds. The 68kDa band observed is likely a splice variant, according to PMID: 16690127.
  • Anti-IRAK-1 antibody [EPR18630] - BSA and Azide free (ab250249)
    Anti-IRAK-1 antibody [EPR18630] - BSA and Azide free (ab250249)

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