Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ZBTB7A antibody [EPR13178(B)] - BSA and Azide free (ab240185)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13178(B)] to ZBTB7A - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-ZBTB7A antibody [EPR13178(B)] - BSA and Azide free
    See all ZBTB7A primary antibodies

  • Description

    Rabbit monoclonal [EPR13178(B)] to ZBTB7A - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IF, IP, WBmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab240185 is the carrier-free version of ab175918 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab240185 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13178(B)

  • Isotype

    IgG

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-ZBTB7A antibody [EPR13178(B)] - BSA and Azide free (ab240185)
    Immunocytochemistry/ Immunofluorescence - Anti-ZBTB7A antibody [EPR13178(B)] - BSA and Azide free (ab240185)

    Immunocytochemistry/Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma cell line) labeling ZBTB7A with purified ab175918 at 1/500 dilution. Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab175918).

  • Immunoprecipitation - Anti-ZBTB7A antibody [EPR13178(B)] - BSA and Azide free (ab240185)
    Immunoprecipitation - Anti-ZBTB7A antibody [EPR13178(B)] - BSA and Azide free (ab240185)

    Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate using labeling ZBTB7A with ab175918 at 1/10 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab175918).

  • Immunocytochemistry/ Immunofluorescence - Anti-ZBTB7A antibody [EPR13178(B)] - BSA and Azide free (ab240185)
    Immunocytochemistry/ Immunofluorescence - Anti-ZBTB7A antibody [EPR13178(B)] - BSA and Azide free (ab240185)

    Immunofluorescent staining of HeLa cells using labeling ZBTB7A with ab175918 at 1/100 dilution

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab175918).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB7A antibody [EPR13178(B)] - BSA and Azide free (ab240185)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB7A antibody [EPR13178(B)] - BSA and Azide free (ab240185)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling ZBTB7A with ab175918 at 1/100 dilution 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab175918).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB7A antibody [EPR13178(B)] - BSA and Azide free (ab240185)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB7A antibody [EPR13178(B)] - BSA and Azide free (ab240185)

    Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue labeling ZBTB7A with ab175918 at 1/100 dilution

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab175918).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-ZBTB7A antibody [EPR13178(B)] - BSA and Azide free (ab240185)
    Anti-ZBTB7A antibody [EPR13178(B)] - BSA and Azide free (ab240185)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-ZBTB7A antibody [EPR13178(B)] - BSA and Azide free (ab240185)

© 2021 Astana Biomed Group LLP. All rights reserved.