Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸ 10 µl 127 315 ₸

Order now and get it on Tuesday March 09, 2021

Anti-ZBTB7A antibody [EPR13178(B)] (ab175918)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13178(B)] to ZBTB7A
Suitable for: WB, IHC-P, ICC/IF, IP
Reacts with: Human

Product name

Anti-ZBTB7A antibody [EPR13178(B)]
See all ZBTB7A primary antibodies
Description

Rabbit monoclonal [EPR13178(B)] to ZBTB7A
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human ZBTB7A aa 500 to the C-terminus. The exact sequence is proprietary.
Database link: O95365
Positive control
- MCF-7, HepG2, K562, Hela cell lysates, human bladder carcinoma tissue, human tonsil tissue, MCF7 and Hela cells.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR13178(B)
Isotype

IgG

Western blot - Anti-ZBTB7A antibody [EPR13178(B)] (ab175918)

All lanes : Anti-ZBTB7A antibody [EPR13178(B)] (ab175918) at 1/10000 dilution

Lane 1 : MCF-7 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : Hela cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 61 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ZBTB7A antibody [EPR13178(B)] (ab175918)

Immunocytochemistry/Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma cell line) labeling ZBTB7A with purified ab175918 at 1/500 dilution. Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
Immunocytochemistry/ Immunofluorescence - Anti-ZBTB7A antibody [EPR13178(B)] (ab175918)

Immunofluorescent staining of HeLa cells using labeling ZBTB7A with ab175918 at 1/100 dilution
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB7A antibody [EPR13178(B)] (ab175918)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling ZBTB7A with ab175918 at 1/100 dilution

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB7A antibody [EPR13178(B)] (ab175918)

Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue labeling ZBTB7A with ab175918 at 1/100 dilution

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunoprecipitation - Anti-ZBTB7A antibody [EPR13178(B)] (ab175918)

Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate using labeling ZBTB7A with ab175918 at 1/10 dilution.
Anti-ZBTB7A antibody [EPR13178(B)] (ab175918)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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