Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ZBTB7A antibody [EPR13178(B)] (ab175918)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13178(B)] to ZBTB7A
  • Suitable for: WB, IHC-P, ICC/IF, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-ZBTB7A antibody [EPR13178(B)]
    See all ZBTB7A primary antibodies

  • Description

    Rabbit monoclonal [EPR13178(B)] to ZBTB7A

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human ZBTB7A aa 500 to the C-terminus. The exact sequence is proprietary.
    Database link: O95365

  • Positive control

    • MCF-7, HepG2, K562, Hela cell lysates, human bladder carcinoma tissue, human tonsil tissue, MCF7 and Hela cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR13178(B)

  • Isotype

    IgG

Images

  • Western blot - Anti-ZBTB7A antibody [EPR13178(B)] (ab175918)
    Western blot - Anti-ZBTB7A antibody [EPR13178(B)] (ab175918)
    All lanes : Anti-ZBTB7A antibody [EPR13178(B)] (ab175918) at 1/10000 dilution

    Lane 1 : MCF-7 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : Hela cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 61 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-ZBTB7A antibody [EPR13178(B)] (ab175918)
    Immunocytochemistry/ Immunofluorescence - Anti-ZBTB7A antibody [EPR13178(B)] (ab175918)

    Immunocytochemistry/Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma cell line) labeling ZBTB7A with purified ab175918 at 1/500 dilution. Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

  • Immunocytochemistry/ Immunofluorescence - Anti-ZBTB7A antibody [EPR13178(B)] (ab175918)
    Immunocytochemistry/ Immunofluorescence - Anti-ZBTB7A antibody [EPR13178(B)] (ab175918)

    Immunofluorescent staining of HeLa cells using labeling ZBTB7A with ab175918 at 1/100 dilution

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB7A antibody [EPR13178(B)] (ab175918)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB7A antibody [EPR13178(B)] (ab175918)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling ZBTB7A with ab175918 at 1/100 dilution 

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB7A antibody [EPR13178(B)] (ab175918)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB7A antibody [EPR13178(B)] (ab175918)

    Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue labeling ZBTB7A with ab175918 at 1/100 dilution

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-ZBTB7A antibody [EPR13178(B)] (ab175918)
    Immunoprecipitation - Anti-ZBTB7A antibody [EPR13178(B)] (ab175918)

    Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate using labeling ZBTB7A with ab175918 at 1/10 dilution.

  • Anti-ZBTB7A antibody [EPR13178(B)] (ab175918)
    Anti-ZBTB7A antibody [EPR13178(B)] (ab175918)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-ZBTB7A antibody [EPR13178(B)] (ab175918)

© 2021 Astana Biomed Group LLP. All rights reserved.