Universal Kinase Assay Kit (Fluorometric) (ab138879)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell Lysate, Purified protein
- Sensitivity: 0.3 µM
Overview
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Product name
Universal Kinase Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Cell Lysate, Purified protein -
Assay type
Enzyme activity -
Sensitivity
Range
1 µM - 300 µMSpecies reactivity
Reacts with: Mammals, Other speciesProduct overview
Universal Kinase Assay Kit (Fluorometric) (ab138879) is based on monitoring ADP formation, which is directly proportional to enzyme phosphotransferase activity and is measured fluorimetrically. This enzyme-coupled kit provides a fast, simple, and homogeneous assay to measure kinase activities. It is a non-radioactive and no wash method to detect the amount of ADP produced from enzyme reaction. Its characteristics of high sensitivity (
Notes
Protein kinases are the enzymes that transfer a phosphate group from a phosphate donor to an acceptor amino acid in a substrate protein. Kinases are of great interest to researchers involved in drug discovery. Most of the commercial protein kinase assay kits are based on monitoring either the phosphopeptide formation or the ATP depletion. For the kinase assay kits that are based on the detection of phosphopeptides, one has to spend time and efforts to identify an optimized peptide substrate while the ATP depletion method suffers various interferences due to the use of luciferase that are inhibited or activated by various biological compounds.
Platform
Microplate readerProperties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 250 tests ADP Assay Buffer 1 x 10ml ADP Sensor Buffer 1 x 5ml ADP Sensor I (Light-sensitive) 1 vial ADP Sensor II 1 x 2.5ml ADP Standard 1 vial DMSO 1 x 100µl -
Research areas
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 250 tests ADP Assay Buffer 1 x 10ml ADP Sensor Buffer 1 x 5ml ADP Sensor I (Light-sensitive) 1 vial ADP Sensor II 1 x 2.5ml ADP Standard 1 vial DMSO 1 x 100µl -
Research areas
Images
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Sample Standard Curve for ADPADP dose response was measured with ab138879 in a solid black 384-well plate using a fluorescence microplate reader. As low as 0.3 µM ADP can be detected with 15, 30 minutes and 1 hour incubation (Z’ factor =0.65).
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Flow DiagramThe Universal Kinase Assay Kit is based on the monitoring of ADP formation, which is directly proportional to enzyme phoshphotransferase activity and is measured fluorimetricaly
