Anti-ErbB2 / HER2 (phospho Y877) antibody (ab47262)
Key features and details
- Rabbit polyclonal to ErbB2 / HER2 (phospho Y877)
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ErbB2 / HER2 (phospho Y877) antibody
See all ErbB2 / HER2 primary antibodies -
Description
Rabbit polyclonal to ErbB2 / HER2 (phospho Y877) -
Host species
Rabbit -
Specificity
Detects endogenous levels of ErbB2 / HER2, only when phosphorylated at Tyrosine 877.
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Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human ErbB2/ HER2.
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Positive control
- IHC-P: Human breast carcinoma tissue; Mouse placenta tissue. WB: HepG2 and HeLa cell lysates; GGF-treated Rat Schwann cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 (phospho Y877) antibody (ab47262)
Staining of phosphorilated ErB 2 on breast carcinoma tissue sections using ab47262 at a 1/50 dilution. Right panel: the antibody was pre-incubated with synthesized phosphopeptide.
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Western blot - Anti-ErbB2 / HER2 (phospho Y877) antibody (ab47262)All lanes : Anti-ErbB2 / HER2 (phospho Y877) antibody (ab47262) (Anti-ErbB2 / HER2 (phospho Y877) antibody)
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate (blocked with the phospho peptide)
Predicted band size: 138 kDaWestern blot analysis of HepG2 and HeLa cell lysate using Anti-ErbB2 / HER2 (phospho Y877) antibody (ab47262).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 (phospho Y877) antibody (ab47262)IHC image of Angiotensin II Type 1 Receptor staining in Mouse Placenta formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47262, 1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - Anti-ErbB2 / HER2 (phospho Y877) antibody (ab47262)Western blot analysis of HeLa cell lysate using Anti-ErbB2 (phospho Y877) antibody (ab47262) at 1/500 dilution.
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Western blot - Anti-ErbB2 / HER2 (phospho Y877) antibody (ab47262) This image is courtesy of an anonymous AbreviewAll lanes : Anti-ErbB2 / HER2 (phospho Y877) antibody (ab47262) at 1/500 dilution
Lane 1 : Schwann cell extract untreated
Lane 2 : Schwann cell extract treated with 10 ng/ml GGF for 30 minutes.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 138 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteLanes on the left hand side show phosphorylation of ErbB2 at tyrosine 877 (ab47262) in rat Schwann cells upon incubation with 10 ng/ml GGF for 30 minutes (lane 2).
Incubation with 10 ng/ml GGF for 30 minutes does not change the amount of total ErbB2 in rat Schwann cells (as shown on right hand side lanes).
