Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸

Order now and get it on Thursday March 04, 2021

Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1046Y] to ErbB2 / HER2 (phospho Y1139)
Suitable for: WB, IP, Dot blot
Reacts with: Human

Product name

Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y]
See all ErbB2 / HER2 primary antibodies
Description

Rabbit monoclonal [EP1046Y] to ErbB2 / HER2 (phospho Y1139)
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human ErbB2/ HER2. The exact sequence is proprietary.
Positive control
- WB: SKBR3 and A431 cell lysates treated with EGF.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
IP	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP1046Y
Isotype

IgG
Research areas

Western blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290)

All lanes : Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290) at 1/500 dilution

Lane 1 : A431 whole cell lysate - untreated
Lane 2 : A431 whole cell lysate - treated with Epidermal Growth Factor (EGF)

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 138 kDa
Observed band size: 185 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking and dilution buffer: 5% NFDM/TBST.
Immunoprecipitation - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290)

ab53290 at 1/70 dilution immunoprecipitating ErbB 2 (phospho Y1139) in A431(human epidermoid carcinoma) whole cell lysate.

Lane 1 (input): A431treated with 100 ng/mL EGF for 10 minutes whole cell lysate 10μg

Lane 2 (+): ab53290 + A431 treated with 100 ng/mL EGF for 10 minutes whole cell lysate

Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab53290 in A431treated with 100 ng/mL EGF for 10 minutes whole cell lysate

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Blocking and Diluting buffer and concentration: 5% NFDM/TBST.
Dot Blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290)

Dot blot analysis of ErbB 2 (pY1139) peptide (Lane 1) and ErbB 2 non-phospho peptide (Lane 2) labelling ErbB 2 (phospho Y1139) with ab53290 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.
Western blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290)

All lanes : Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290) at 1/1000 dilution

Lane 1 : SKBR3 cell lysate
Lane 2 : SKBR3 cell lysate - treated with EGF

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 138 kDa
Observed band size: 185 kDa why is the actual band size different from the predicted?
Western blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290) This image is courtesy of an Abreview by Surekha Pimple.

All lanes : Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290) at 1/2000 dilution

Lane 1 : HTB-2O Human breast ductal carcinoma lysate untreated
Lanes 2-9 : HTB-2O Human breast ductal carcinoma lysate treated with HER-2 inhibitor

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP-conjugated Mouse anti-Rabbit monoclonal at 1/20000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 138 kDa

Exposure time: 5 seconds

Lanes 2-9 are treated with an increasing concentration of HER2 inhibitor.
Blocking performed with 5% Milk for 1 hour at room temperature.
Antibody diluted in CPPT and incubated for 16 hours at 4°C.
Performed under denaturing conditions.
See Abreview
Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290)

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