Anti-ERO1L antibody [EPR12474] (ab177156)
![Anti-ERO1L antibody [EPR12474] (ab177156)](https://www.abcam.com/ps/products/177/ab177156/Images/ab177156-205164-anti-ero1l-antibody-epr12474-western-blot.jpg "Anti-ERO1L antibody [EPR12474] (ab177156)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12474] to ERO1L
- Suitable for: WB, IHC-P, Flow Cyt, IP
- Reacts with: Human
Overview
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Product name
Anti-ERO1L antibody [EPR12474]
See all ERO1L primary antibodies -
Description
Rabbit monoclonal [EPR12474] to ERO1L -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanIP HumanWB Human
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Immunogen
Synthetic peptide within Human ERO1L aa 1-100. The exact sequence is proprietary.
Database link: Q96HE7 -
Positive control
- HepG2, 293T, MCF7 and HeLa whole cell lysate (ab150035). Human colon tissue. Permeabilized HeLa cells.
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12474 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ERO1L antibody [EPR12474] (ab177156)All lanes : Anti-ERO1L antibody [EPR12474] (ab177156) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 54 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERO1L antibody [EPR12474] (ab177156)](https://www.abcam.com/ps/products/177/ab177156/Images/ab177156-205165-anti-ero1l-antibody-epr12474-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERO1L antibody [EPR12474] (ab177156)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling ERO1L with ab177156 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-ERO1L antibody [EPR12474] (ab177156)](https://www.abcam.com/ps/products/177/ab177156/Images/ab177156-205167-anti-ero1l-antibody-epr12474-immunoprecipitation.jpg)
Immunoprecipitation - Anti-ERO1L antibody [EPR12474] (ab177156)Western blot analysis on immunoprecipitation pellet from (Lane 1) HepG2 cell lysate or (Lane 2) 1 X PBS (negative control) using ab177156 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
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![Flow Cytometry - Anti-ERO1L antibody [EPR12474] (ab177156)](https://www.abcam.com/ps/products/177/ab177156/Images/ab177156-205166-anti-ero1l-antibody-epr12474-flow-cytometry.jpg)
Flow Cytometry - Anti-ERO1L antibody [EPR12474] (ab177156)Flow cytometric analysis of permeabilized HeLa cells labeling ERO1L with ab177156 at 1/10 dilution (red), or a rabbit IgG (negative) (green).
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![Anti-ERO1L antibody [EPR12474] (ab177156)](https://www.abcam.com/ps/products/177/ab177156/Images/ab177156-5-benefits-of-recombinant-antibodies.png)
Anti-ERO1L antibody [EPR12474] (ab177156)
