Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Thrombospondin 1 antibody [EPR22928-10] (ab263905)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22928-10] to Thrombospondin 1
  • Suitable for: WB, IHC-Fr, IP, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Thrombospondin 1 antibody [EPR22928-10]
    See all Thrombospondin 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR22928-10] to Thrombospondin 1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    Rat
    IHC-P
    Mouse
    Rat
    Human
    IP
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: 3T3-L1 starved with 0.4% serum for 24 hours, then cultivated with 15% serum for 6 hours. whole cell lysate. Mouse platelet lysate. IHC-P: Human spleen and bone marrow. Mouse and rat spleen tissue. IP: Mouse platelet cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22928-10

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Thrombospondin 1 antibody [EPR22928-10] (ab263905)
    Western blot - Anti-Thrombospondin 1 antibody [EPR22928-10] (ab263905)
    Anti-Thrombospondin 1 antibody [EPR22928-10] (ab263905) at 1/1000 dilution + Mouse platelet lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/25000 dilution

    Predicted band size: 129 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 27588705).

    Exposure time: 1 second.

    Observed MW: 180 kDa.

  • Western blot - Anti-Thrombospondin 1 antibody [EPR22928-10] (ab263905)
    Western blot - Anti-Thrombospondin 1 antibody [EPR22928-10] (ab263905)
    All lanes : Anti-Thrombospondin 1 antibody [EPR22928-10] (ab263905) at 1/1000 dilution

    Lane 1 : 3T3-L1 (mouse embryonic fibroblast) starved with 0.4% serum for 30 hours whole cell lysate
    Lane 2 : 3T3-L1 starved with 0.4% serum for 24 hours, then cultivated with 15% serum for 6 hours. whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/25000 dilution

    Predicted band size: 129 kDa
    Observed band size: 180 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    TSP 1 is a serum-responsive gene. Its express is elevated in quiescent 3T3 with serum addition.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 8422970, 27588705).

    Exposure time: 3 minutes.

  • Immunoprecipitation - Anti-Thrombospondin 1 antibody [EPR22928-10] (ab263905)
    Immunoprecipitation - Anti-Thrombospondin 1 antibody [EPR22928-10] (ab263905)

    Thrombospondin 1 was immunoprecipitated from 0.35 mg mouse platelet lysate 10µg with ab263905 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab263905 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse platelet lysate 10µg

    Lane 2: ab263905 IP in mouse platelet lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab263905 in mouse platelet lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 mins.

     

  • Immunohistochemistry (Frozen sections) - Anti-Thrombospondin 1 antibody [EPR22928-10] (ab263905)
    Immunohistochemistry (Frozen sections) - Anti-Thrombospondin 1 antibody [EPR22928-10] (ab263905)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat spleen tissue labeling Thrombospondin 1 with ab263905 at 1/500 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive staining on rat splenic megakaryocytes and platelet (PMID: 28239144) is observed. Nuclear counterstain DAPI.

    Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Frozen sections) - Anti-Thrombospondin 1 antibody [EPR22928-10] (ab263905)
    Immunohistochemistry (Frozen sections) - Anti-Thrombospondin 1 antibody [EPR22928-10] (ab263905)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse spleen tissue labeling Thrombospondin 1 with ab263905 at 1/500 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive staining on mouse splenic megakaryocytes and platelet (PMID: 28239144) is observed. Nuclear counterstain DAPI.

    Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin 1 antibody [EPR22928-10] (ab263905)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin 1 antibody [EPR22928-10] (ab263905)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Thrombospondin 1 with ab263905 at 1/2000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on the megakaryocytes and platelets in the rat spleen. The section was incubated with ab263905 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin 1 antibody [EPR22928-10] (ab263905)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin 1 antibody [EPR22928-10] (ab263905)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Thrombospondin 1 with ab263905 at 1/2000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on the megakaryocytes and platelets in the mouse spleen. The section was incubated with ab263905 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin 1 antibody [EPR22928-10] (ab263905)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin 1 antibody [EPR22928-10] (ab263905)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Thrombospondin 1 with ab263905 at 1/5000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on the platelets in the human spleen (PMID: 28239144). The section was incubated with ab263905 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin 1 antibody [EPR22928-10] (ab263905)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin 1 antibody [EPR22928-10] (ab263905)

    Immunohistochemical analysis of paraffin-embedded human bone marrow tissue labeling Thrombospondin 1 with ab263905 at 1/5000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on the megakaryocytes in the human bone marrow (PMID: 28239144). The section was incubated with ab263905 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Anti-Thrombospondin 1 antibody [EPR22928-10] (ab263905)
    Anti-Thrombospondin 1 antibody [EPR22928-10] (ab263905)

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