Immunofluorescent staining of Methanol-fixed MCF-7 cells labeling ErbB2 / HER2 with ab212339 followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Reddot (Red).

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue labeling ErbB 2 with ab212339 at 1 µg/ml.

Flow Cytometric analysis of human trypsinized SK-BR-3 (human mammary gland adenocarcinoma cell line) cells labeling ErbB2 / HER2 with ab212339 followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

This data was produced with ab187288, the same antibody in a different formulation with BSA and Azide.
ab187288 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue labeling ErbB 2 with ab212339 at 1 µg/ml.

Immunofluorescent staining of PFA-fixed MCF-7 cells labeling ErbB2 / HER2 with ab212339 followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Reddot (Red).

Flow Cytometric analysis of human trypsinized MCF7 (human breast adenocarcinoma cell line) cells labeling ErbB2 / HER2 with ab212339 followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).