Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] (ab108371)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2324Y] to ErbB2 / HER2 (phospho Y877)
- Suitable for: WB, IHC-P, Dot blot, Flow Cyt, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y]
See all ErbB2 / HER2 primary antibodies -
Description
Rabbit monoclonal [EP2324Y] to ErbB2 / HER2 (phospho Y877) -
Host species
Rabbit -
Specificity
ab108371 detects ErbB2 / HER2 phosphorylated at tyrosine 877.
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Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa cell lysate, SKBR-3 cell lysate, Human breast ductal infiltrating carcinoma tissue, SKBR-3 cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA disodium salt, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP2324Y -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] (ab108371)
ab108371, at 1/250, staining Human ErbB2 / HER2 (phospho Y877) in Human breast ductal infiltrating carcinoma tissue by immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] (ab108371) at 1/1000 dilution
Lane 1 : A431 cell lysate - untreated
Lane 2 : A431 cell lysate - treated with Epidermal Growth Factor (EGF)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/100000 dilution
Predicted band size: 138 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking and dilution buffer: 5% NFDM/TBST.
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Flow Cytometry analysis of A431 (human epidermoid carcinoma) untreated/treated with 200 ng/ml EGF for 15 minutes labeling ErbB2 / HER2 with purified ab108371 at 1/20. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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Dot blot analysis of ErbB 2 (pY877) peptide (Lane 1) and ErbB2 / HER2 non-phospho peptide (Lane 2) labelling ErbB 2 (phospho Y877) with ab108371 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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All lanes : Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] (ab108371) at 1/500 dilution
Lane 1 : HeLa cell lysate
Lane 2 : EGF treated HeLa cell lysate
Lane 3 : SKBR-3 cell lysate
Lane 4 : EGF treated SKBR-3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 138 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] (ab108371)
ab108371, at 1/50 dilution, staining Human ErbB2 / HER2 (phospho Y877) in SKBR-3 cells by immunofluorescence.
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