Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

Order now and get it on Tuesday March 02, 2021

Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] (ab108371)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP2324Y] to ErbB2 / HER2 (phospho Y877)
Suitable for: WB, IHC-P, Dot blot, Flow Cyt, ICC/IF
Reacts with: Human

Product name

Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y]
See all ErbB2 / HER2 primary antibodies
Description

Rabbit monoclonal [EP2324Y] to ErbB2 / HER2 (phospho Y877)
Host species

Rabbit
Specificity

ab108371 detects ErbB2 / HER2 phosphorylated at tyrosine 877.

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- HeLa cell lysate, SKBR-3 cell lysate, Human breast ductal infiltrating carcinoma tissue, SKBR-3 cells.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA disodium salt, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP2324Y
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] (ab108371)

ab108371, at 1/250, staining Human ErbB2 / HER2 (phospho Y877) in Human breast ductal infiltrating carcinoma tissue by immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] (ab108371)

All lanes : Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] (ab108371) at 1/1000 dilution

Lane 1 : A431 cell lysate - untreated
Lane 2 : A431 cell lysate - treated with Epidermal Growth Factor (EGF)

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/100000 dilution

Predicted band size: 138 kDa
Observed band size: 180 kDa
why is the actual band size different from the predicted?

Exposure time: 1 minute

Blocking and dilution buffer: 5% NFDM/TBST.
Flow Cytometry - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] (ab108371)

Flow Cytometry analysis of A431 (human epidermoid carcinoma) untreated/treated with 200 ng/ml EGF for 15 minutes labeling ErbB2 / HER2 with purified ab108371 at 1/20. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Dot Blot - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] (ab108371)

Dot blot analysis of ErbB 2 (pY877) peptide (Lane 1) and ErbB2 / HER2 non-phospho peptide (Lane 2) labelling ErbB 2 (phospho Y877) with ab108371 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.
Western blot - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] (ab108371)

All lanes : Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] (ab108371) at 1/500 dilution

Lane 1 : HeLa cell lysate
Lane 2 : EGF treated HeLa cell lysate
Lane 3 : SKBR-3 cell lysate
Lane 4 : EGF treated SKBR-3 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 138 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] (ab108371)

ab108371, at 1/50 dilution, staining Human ErbB2 / HER2 (phospho Y877) in SKBR-3 cells by immunofluorescence.
Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] (ab108371)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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