Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody [EPR1047] (ab91633)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1047] to ErbB2 / HER2 (phospho Y1221 + Y1222)
- Suitable for: WB, Dot blot
- Reacts with: Human
Overview
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Product name
Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody [EPR1047]
See all ErbB2 / HER2 primary antibodies -
Description
Rabbit monoclonal [EPR1047] to ErbB2 / HER2 (phospho Y1221 + Y1222) -
Host species
Rabbit -
Specificity
ab91633 detects ErbB 2 phosphorylated at tyrosine 1222. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
corresponding to Human ErbB2/ HER2. Phosphospecific peptide (UniProt ID: P04626).
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Positive control
- SKBR3 cell lysate
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR1047 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody [EPR1047] (ab91633) at 1/5000 dilution
Lane 1 : SKBR3 cell lysate was untreated
Lane 2 : SKBR3 cell lysate was treated with Lambda phosphatase.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/1000 dilution
Predicted band size: 138 kDa
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Primary antibody dilution: 1/1000
Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated
Secondary antibody dilution: 1/2500
Blocking & dilution buffer: 5% NFDM/TBST
Lane 1 sample: ErB 2 (pY1221/pY1222) phospho peptide
Lane 2 sample: ErB 2 non-phospho peptide
Exposure time: 3 minutes
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