Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1045Y] to ErbB2 / HER2
  • Suitable for: WB, IP, ICC/IF, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-ErbB2 / HER2 antibody [EP1045Y]
    See all ErbB2 / HER2 primary antibodies

  • Description

    Rabbit monoclonal [EP1045Y] to ErbB2 / HER2

  • Host species

    Rabbit

  • Specificity

    ab134182 detects ErbB 2 phosphorylated at Tyr1248 as well as unphosphorylated ErbB 2.

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    within Human ErbB2/ HER2 aa 1200 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P04626

  • Positive control

    • WB: HeLa, SKBR-3 cell lysate; IHC-P: Human breast carcinoma tissue; ICC/IF: SKBR cells; IP: HeLa.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
    Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
    Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182) at 1/1000 dilution + 4T1(Mouse mammary gland carcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 137 kDa
    Observed band size: 180 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling EErbB2 / HER2 with Purified ab134182 at 1:1600 dilution (0.68 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunoprecipitation - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
    Immunoprecipitation - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)

    ab134182 (purified) at 1:30 dilution (2µg) immunoprecipitating ErbB2 / HER2 in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab134182 & HeLa whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab134182 in HeLa whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
    Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
    Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182) at 1/10000 dilution (purified) + SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 137 kDa



    Blocking and diluting buffer : 5% NFDM/TBST
  • Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
    Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
    Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182) at 1/1000 dilution (purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 137 kDa



    Blocking and diluting buffer : 5% NFDM/TBST
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling ErbB2 / HER2 with ab134182 at 1/100 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer, pH 9.0).

  • Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
    Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)

    Immunofluorescent analysis of SKBR cells labelling ErbB2 / HER2 with ab134182 at 1/250 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
    Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)

    Immunocytochemistry/Immunofluorescence analysis of SK-BR-3 (human mammary gland adenocarcinoma) labelling ErbB2 / HER2 with purified ab134182 at 1/125. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

    Control: PBS only

  • Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
    Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
    Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182) at 1/10000 dilution + SKBR-3 cell lysate at 10 µg

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 137 kDa

  • OI-RD Scanning - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
    OI-RD Scanning - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
    Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)

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