Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] (ab75873)
![Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] (ab75873)](https://www.abcam.com/ps/products/75/ab75873/Images/ab75873-91023-anti-erbb-2-phospho-y877-antibody-epr2324y-western-blot.gif "Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] (ab75873)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2324Y] to ErbB2 / HER2 (phospho Y877)
- Suitable for: Dot blot, WB, Flow Cyt, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y]
See all ErbB2 / HER2 primary antibodies -
Description
Rabbit monoclonal [EPR2324Y] to ErbB2 / HER2 (phospho Y877) -
Host species
Rabbit -
Specificity
ab75873 detects ErbB2 / HER2 phosphorylated at tyrosine 877.
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Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB Human
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa and SKBR3 cell lysates treated with EGF. SKBR3 cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 8.30 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2324Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] (ab75873)All lanes : Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] (ab75873) at 1/1000 dilution
Lane 1 : HeLa cell lysate, untreated
Lane 2 : HeLa cell lysate, treated with EGF
Lane 3 : SKBR3 cell lysate, untreated
Lane 4 : SKBR3 cell lysate, treated with EGF
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 138 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
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![Dot Blot - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] (ab75873)](https://www.abcam.com/ps/products/75/ab75873/Images/ab75873-255422-anti-erbb-2-phospho-y877-antibody-epr2324y-dot-blot.jpg)
Dot Blot - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] (ab75873)Dot blot analysis of ErbB 2 (pY877) peptide (Lane 1) and ErbB 2 non-phospho peptide (Lane 2) labelling ErbB 2 (phospho Y877) with ab75873 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Flow Cytometry - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] (ab75873)](https://www.abcam.com/ps/products/75/ab75873/Images/ab75873-276594-anti-erbb-2-phospho-y877-antibody-epr2324y-flow-cytometry.jpg)
Flow Cytometry - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] (ab75873)Flow Cytometry analysis of A431 (human epidermoid carcinoma) untreated/treated with 200 ng/ml EGF for 15 minutes labeling ErbB 2 with unpurified ab75873 at 1/20 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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![Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] (ab75873)](https://www.abcam.com/ps/products/75/ab75873/Images/ab75873-91022-anti-erbb-2-phospho-y877-antibody-epr2324y-immunofluorescence.gif)
Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] (ab75873)ab75873 at 1/50 dilution staining ErbB 2 in SKBR3 cells by Immunofluorescence. -
![OI-RD Scanning - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] (ab75873)](https://www.abcam.com/ps/products/75/ab75873/Images/ab75873-91021-anti-erbb-2-phospho-y877-antibody-epr2324y.jpg)
OI-RD Scanning - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] (ab75873) -
![Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] (ab75873)](https://www.abcam.com/ps/products/75/ab75873/Images/ab75873-6-benefits-of-recombinant-antibodies.png)
Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] (ab75873)
