Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] (ab75873)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2324Y] to ErbB2 / HER2 (phospho Y877)
  • Suitable for: Dot blot, WB, Flow Cyt, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y]
    See all ErbB2 / HER2 primary antibodies

  • Description

    Rabbit monoclonal [EPR2324Y] to ErbB2 / HER2 (phospho Y877)

  • Host species

    Rabbit

  • Specificity

    ab75873 detects ErbB2 / HER2 phosphorylated at tyrosine 877.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa and SKBR3 cell lysates treated with EGF. SKBR3 cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] (ab75873)
    Western blot - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] (ab75873)
    All lanes : Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] (ab75873) at 1/1000 dilution

    Lane 1 : HeLa cell lysate, untreated
    Lane 2 : HeLa cell lysate, treated with EGF
    Lane 3 : SKBR3 cell lysate, untreated
    Lane 4 : SKBR3 cell lysate, treated with EGF

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 138 kDa
    Observed band size: 180 kDa
    why is the actual band size different from the predicted?

  • Dot Blot - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] (ab75873)
    Dot Blot - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] (ab75873)

    Dot blot analysis of ErbB 2 (pY877) peptide (Lane 1) and ErbB 2 non-phospho peptide (Lane 2) labelling ErbB 2 (phospho Y877) with ab75873 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Flow Cytometry - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] (ab75873)
    Flow Cytometry - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] (ab75873)

    Flow Cytometry analysis of A431 (human epidermoid carcinoma) untreated/treated with 200 ng/ml EGF for 15 minutes labeling ErbB 2 with unpurified ab75873 at 1/20 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] (ab75873)
    Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] (ab75873)
    ab75873 at 1/50 dilution staining ErbB 2 in SKBR3 cells by Immunofluorescence.
  • OI-RD Scanning - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] (ab75873)
    OI-RD Scanning - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] (ab75873)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] (ab75873)
    Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] (ab75873)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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