Anti-UBE4B antibody [EPR7471] (ab126759)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7471] to UBE4B
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-UBE4B antibody [EPR7471]
See all UBE4B primary antibodies -
Description
Rabbit monoclonal [EPR7471] to UBE4B -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Synthetic peptide within Human UBE4B aa 1-100. The exact sequence is proprietary.
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Positive control
- WB: 293T, Raji, SK OV 3, L929, L6, RAW264.7, PC12, NIH 3T3, HeLa, and K562 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7471 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-UBE4B antibody [EPR7471] (ab126759) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : Raji cell lysate
Lane 3 : SK OV 3 cell lysate
Lane 4 : L929 cell lysate
Lane 5 : L6 cell lysate
Lane 6 : RAW264.7 cell lysate
Lane 7 : PC12 cell lysate
Lane 8 : NIH 3T3 cell lysate
Lane 9 : HeLa cell lysate
Lane 10 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution
Predicted band size: 146 kDa
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Western blot analysis of Human 293NT whole cell lysate labeling UBE4B with ab126759 at 1/2000. A HRP-conjugated goat anti-rabbit IgG polyclonal (1/20000) was used as the secondary antibody.
siRNA agains UBE4B was used to show for antibody specificity.
Unspecific siRNA (siLUC) was used as a negative control.
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