Anti-Rb (phospho S795) antibody (ab47474)
Key features and details
- Rabbit polyclonal to Rb (phospho S795)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Rb (phospho S795) antibody
See all Rb primary antibodies -
Description
Rabbit polyclonal to Rb (phospho S795) -
Host species
Rabbit -
Specificity
This antibody detects endogenous levels of Rb only when phosphorylated at serine 795 -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
-
Immunogen
A synthesized phosphopeptide derived from human Rb around the phosphorylation site of serine 795 (P-S-SP-P-L)
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Rb (phospho S795) antibody (ab47474)All lanes : Anti-Rb (phospho S795) antibody (ab47474) at 1/500 dilution
Lane 1 : Extracts from K562 cells treated with 10% serum after 48 hours of starvation
Lane 2 : Extracts from K562 cells
Lysates/proteins at 30 µg per lane.
Predicted band size: 106 kDa
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S795) antibody (ab47474)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue in the presence (right) and absence (left) of synthetic peptide, using ab47474 at a 1/50 dilution.
