Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR182(N)] to Rb (phospho S780)
Suitable for: Dot blot, IHC-P, WB
Reacts with: Human

Product name

Anti-Rb (phospho S780) antibody [EPR182(N)]
See all Rb primary antibodies
Description

Rabbit monoclonal [EPR182(N)] to Rb (phospho S780)
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Rb aa 750-850 (phospho S780) (Cysteine residue). The exact sequence is proprietary.
Database link: P06400
Positive control
- Jurkat cell lysate; Human thyroid gland carcinoma and testis tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR182(N)
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Rb (phospho S780) with ab173289 at 1/100 dilution,followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Nucleus staining on normal human colon.

Counter stained with Hematoxylin.

Secondary antibody only control:Used PBS instead of primary antibody,secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289)

All lanes : Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289) at 1/50000 dilution

Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) serum starved for 44 h. Whole cell lysates
Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) serum starved for 24 h. Then 10% FBS incubated for 20 h. Whole cell lysates
Lane 3 : Jurkat (Human T cell leukemia T lymphocyte) serum starved for 24 h. Then 10% FBS incubated for 20 h. Whole cell lysates Then the membrane was incubated with phosphatase.

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 106 kDa
Observed band size: 106 kDa

Exposure time: 1 minute

Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Western blot - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289)

Lane 1 : Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289) at 1/20000 dilution
Lane 2 : Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289)

Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 2 : K562(Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate, serum starved for 2 days

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 106 kDa

Blocking/Dilution buffer:5% NFDM/TBST
Exposure time:1 minute
Western blot - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289)

All lanes : Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289) at 1/5000 dilution

Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 2 : Calyculin A treated K562(Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 106 kDa

Blocking/Dilution buffer:NFDM/TBST
Ab173289: anti- Rb (phospho S780);
Ab184796: anti- Rb (phospho S807);
Ab181616: anti-Rb
Dot Blot - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289)

Dot blot analysis of Rb (phospho S780) peptide (Lane 1),Rb(non-phospho) peptide (Lane 2) with ab173289 at a dilution of 1/1000.ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/1000.
Blocking and dilution buffer:5% NFDM/TBST.
Exposure time:3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289)

Immunohistochemical analysis of paraffin-embedded Lung squamous cell carcinoma labeling Rb (phospho S780) with ab173289 at 1/100 dilution,followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Nucleus staining on tumor cells of lung squamous cell carcinoma.

Counter stained with Hematoxylin.

Secondary antibody only control:Used PBS instead of primary antibody,secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289)

All lanes : Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289) at 1/1000 dilution

Lane 1 : Jurkat cell lysate
Lane 2 : Jurkat cell lysate treated with Lambda Phosphatase

Lysates/proteins at 10 µg per lane.

Predicted band size: 106 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289)

Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue labeling Rb with ab173289 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Rb with ab173289 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289)

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