Antibodies, Proteins, Kits and Reagents for Life Science

251 280 ₸ Product size

100 µl 251 280 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (ab68136)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP2387AY] to Rb2 p130 (phospho S952)
Suitable for: WB, IHC-P
Reacts with: Mouse, Human

Product name

Anti-Rb2 p130 (phospho S952) antibody [EP2387AY]
See all Rb2 p130 primary antibodies
Description

Rabbit monoclonal [EP2387AY] to Rb2 p130 (phospho S952)
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Human
WB	Mouse Human

See all applications and species data

Immunogen

corresponding to Human Rb2 p130.
Positive control
- WB: Jurkat and RAW 264.7 cell lysate; mouse heart tissue lysate IHC-P: human breast and lung carcinoma tissue, Mouse cerebrum
General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP2387AY
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (ab68136)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung cancer with alkaline phosphatase treatment (image A) and treated without alkaline phosphatase (image B) tissue sections labeling Rb2 p130 with purified ab68136 at 1/600 dilution (0.36 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (ab68136)

All lanes : Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (ab68136) at 1/2000 dilution (Purified)

Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates. Then the membrane was incubated with phosphatase
Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 128 kDa
Observed band size: 128 kDa
Western blot - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (ab68136)

All lanes : Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (ab68136) at 1/5000 dilution (Purified)

Lane 1 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates. Then the membrane was incubated with phosphatase
Lane 2 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 128 kDa
Observed band size: 128 kDa
Western blot - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (ab68136)

All lanes : Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (ab68136) at 1/2000 dilution (Purified)

Lane 1 : Mouse heart lysates. Then the membrane was incubated with phosphatase
Lane 2 : Mouse heart lysates

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 128 kDa
Observed band size: 128 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (ab68136)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum with alkaline phosphatase treatment (image A) and treated without alkaline phosphatase (image B) tissue sections labeling Rb2 p130 with purified ab68136 at 1/600 dilution (0.36 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (ab68136)

All lanes : Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (ab68136) at 1/500000 dilution (unpurified)

Lane 1 : Jurkat cell lysate, untreated
Lane 2 : Jurkat cell lysate, treated with AP

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP-labeled goat anti-mouse at 1/2000 dilution

Predicted band size: 128 kDa
Observed band size: 128 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (ab68136)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab68136 (unpurified) at a dilution of 1/100.
Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (ab68136)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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