Antibodies, Proteins, Kits and Reagents for Life Science

335 040 ₸ Product size

100 µl 335 040 ₸ 10 µl 134 016 ₸

Order now and get it on Tuesday March 02, 2021

Anti-GSK3 beta (phospho S9) antibody [EPR2286Y] (ab75814)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2286Y] to GSK3 beta (phospho S9)
Suitable for: Dot blot, WB, IHC-P, ICC/IF
Reacts with: Human

Product name

Anti-GSK3 beta (phospho S9) antibody [EPR2286Y]
See all GSK3 beta primary antibodies
Description

Rabbit monoclonal [EPR2286Y] to GSK3 beta (phospho S9)
Host species

Rabbit
Specificity

ab75814 only detects GSK3 beta phosphorylated on Serine 9.

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

A phospho-specific peptide corresponding to residues surrounding Serine 9 of human GSK3 beta
Positive control
- 293T cell lysates, untreated or treated with Calyculin A and human ovarian carcinoma tissue.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR2286Y
Isotype

IgG
Research areas

Western blot - Anti-GSK3 beta (phospho S9) antibody [EPR2286Y] (ab75814)

All lanes : Anti-GSK3 beta (phospho S9) antibody [EPR2286Y] (ab75814) at 1/20000 dilution

Lane 1 : Untreated HeLa cell lysate
Lane 2 : HeLa cells were starved for 3 hours then treated with Calyculin A (100nM) for 30 minutes.
Lane 3 : HeLa cells were starved for 3 hours then treated with Calyculin A (100nM) for 30 minutes. The membrane was treated with alkaline phosphatase.

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat anti-rabbit IgG H&L (HRP))

Predicted band size: 47 kDa
Observed band size: 47 kDa

Exposure time: 5 seconds

Blocking buffer: 5% NFDM/TBST for the first 3 lanes, 2% BSA/TBST for the second 3 lanes.

Dilution buffer: 5% NFDM/TBST for the first 3 lanes, 2% BSA/TBST for the second 3 lanes.
Immunocytochemistry/ Immunofluorescence - Anti-GSK3 beta (phospho S9) antibody [EPR2286Y] (ab75814)

Cell line: HeLa (human cervix adenocarcinoma)

Target: Ab75814 anti-GSK3 beta (phospho S9)

Secondary: ab150077 AlexaFluor®488 Goat anti-Rabbit

Fixative: 4% PFA

Permeabilization: 0.1% Triton-X

Nuclear counter stain: DAPI

Confocal image showed increased cytoplasmic staining after Calyculin A (CA) (ab141784 100ng/ml, 10min) treatment on Hela cells. The LP treatment decreased the increased cytoplasmic staining caused by CA.

Ab32391 was used as a control for ab75814. The results showed cytoplasmic staining on untreated, CA and CA+LP treated Hela cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSK3 beta (phospho S9) antibody [EPR2286Y] (ab75814)

Immunohistochemical analysis of paraffin-embedded human breast tissue labelled with untreated ab75814 (phospho) (top-left) at a dilution of 1/1000, alkaline phosphatase treated ab75814 (phospho) (top-right) at a dilution of 1/1000, untreated ab32391 (bottom-left) at a dilution of 1/1000 and alkaline phophatase treated ab32391 (bottom-right) at a dilution of 1/1000. Ab97051 was used as secondary antibody at a dilution of 1/500 and counterstained with hematoxylin.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Dot Blot - Anti-GSK3 beta (phospho S9) antibody [EPR2286Y] (ab75814)

Dot blot analysis of GSK3 beta (pS9) peptide (Lane 1) and GSK3 beta non-phospho peptide (Lane 2) labelling GSK3 (pS9) with purified ab75814 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSK3 beta (phospho S9) antibody [EPR2286Y] (ab75814)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labelled with untreated ab75814 (top-left) at a dilution of 1/1000, alkaline phosphatase treated ab75814 (top-right) at a dilution of 1/1000, untreated ab32391 (bottom-left) at a dilution of 1/1000 and alkaline phophatase treated ab32391 (bottom-right) at a dilution of 1/1000. Ab97051 was used as secondary antibody at a dilution of 1/500 and counterstained with hematoxylin.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-GSK3 beta (phospho S9) antibody [EPR2286Y] (ab75814)

All lanes : Anti-GSK3 beta (phospho S9) antibody [EPR2286Y] (ab75814) at 1/20000 dilution

Lane 1 : 293T cell lysates, untreated
Lane 2 : 293T cell lysates, treated with Calyculin A

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 47 kDa
Observed band size: 47 kDa
Anti-GSK3 beta (phospho S9) antibody [EPR2286Y] (ab75814)

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