HRP Anti-GSK3 beta antibody [Y174] (ab196911)
![HRP Anti-GSK3 beta antibody [Y174] (ab196911)](https://www.abcam.com/ps/products/196/ab196911/Images/ab196911-327961-ab196911GR2035601GSK3B20min.jpg "HRP Anti-GSK3 beta antibody [Y174] (ab196911)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [Y174] to GSK3 beta
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-GSK3 beta antibody [Y174]
See all GSK3 beta primary antibodies -
Description
HRP Rabbit monoclonal [Y174] to GSK3 beta -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A431 whole cell lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Y174 -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-GSK3 beta antibody [Y174] (ab196911)All lanes : HRP Anti-GSK3 beta antibody [Y174] (ab196911) at 1/5000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : GSK3B knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 46 kDa
Exposure time: 20 minutesab196911 was shown to recognize GSK3 beta in wild-type HAP1 cells as signal was lost at the expected MW in GSK3B knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GSK3B knockout samples were subjected to SDS-PAGE. Ab196911 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
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![Western blot - HRP Anti-GSK3 beta antibody [Y174] (ab196911)](https://www.abcam.com/ps/products/196/ab196911/Images/ab196911-238289-anti-gsk3-beta-antibody-y174-hrp-western-blot.jpg)
Western blot - HRP Anti-GSK3 beta antibody [Y174] (ab196911)HRP Anti-GSK3 beta antibody [Y174] (ab196911) at 1/5000 dilution + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Exposure time: 2 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab196911 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-GSK3 beta antibody [Y174] (ab196911)](https://www.abcam.com/ps/products/196/ab196911/Images/ab196911-3-benefits-of-recombinant-antibodies.png)
HRP Anti-GSK3 beta antibody [Y174] (ab196911)
