Recombinant human DLL4 protein (Active) (ab219667)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 92% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 5% Trehalose, PBS
Lyophilized from 0.22 µm filtered solution.
5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 200 µg/ml.
Images
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Functional Studies - Recombinant human DLL4 protein (Active) (ab219667)
Immobilized Mouse Notch-1 Protein ECD, Fc Tag at 2 μg/mL (100 μL/well) can bind ab219667 with a linear range of 0.039-0.313 μg/mL.
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Functional Studies - Recombinant human DLL4 protein (Active) (ab219667)Immobilized ab219667 at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human DLL4 Antibody, Human IgG1 with a linear range of 0.2-4 ng/mL.
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SDS-PAGE - Recombinant Human DLL4 protein (His tag) (ab219667)ab219667 on SDS-PAGE under reducing (R) conditions.
The gel was stained overnight with Coomassie Blue.
The purity of the protein is greater than 92%.
The protein migrates as 65 kDa under reducing (R) conditions due to glycosylation.
